製品の概要

  • 製品名pan Caspase (active) Red Staining Kit
    Caspase キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's pan Caspase (active) Red Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes the caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent in situ marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Activation of caspases plays a central role in apoptosis.

  • アプリケーション適用あり: FM, Flow Cyt, Functional Studiesmore details

製品の特性

  • 関連性Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • 細胞内局在Cytoplasmic

アプリケーション

Our Abpromise guarantee covers the use of ab65616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.

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pan Caspase (active) Red Staining Kit (ab65616) 使用論文

ab65616 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"