Anti-pan Cadherin 抗体 - Plasma Membrane Marker (ab16505)

製品の概要

  • 製品名Anti-pan Cadherin antibody - Plasma Membrane Marker
    pan Cadherin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to pan Cadherin - Plasma Membrane Marker
  • アプリケーション適用あり: ICC/IF, ICC, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
    交差が予測される動物種: Chicken, Cow, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human pan Cadherin.

    (Peptide available as ab17098.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; 3T3.

      This antibody gave a positive signal in the following tissue lysates: Mouse Heart Normal; Mouse Muscle normal; Human Heart Normal; Rat Heart Normal.

      This antibody gave a positive signal in the following cell lines: HeLa.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Liver Normal.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 2 µg/ml.

A diffuse signal is seen throughout the cells if higher concentrations are used (5-10µg/ml). We have had reports that the antibody works less well in this application in murine (3T3) cells. 

ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

Anti-pan Cadherin antibody - Plasma Membrane Marker 画像

  • All lanes : Anti-pan Cadherin antibody - Plasma Membrane Marker (ab16505) at 1 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 125-140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,65 kDa,75 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab16505 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16505, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • IHC image of pan Cadherin staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16505, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-pan Cadherin antibody - Plasma Membrane Marker (ab16505) at 1 µg/ml

    Lane 1 : Mouse heart
    Lane 2 : HeLa cell lysate
    Lane 3 : 3T3 cell lysate
    Lane 4 : Mouse muscle
    Lane 5 : Human heart
    Lane 6 : Mouse heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml
    Lane 7 : HeLa cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml
    Lane 8 : 3T3 cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml
    Lane 9 : Mouse muscle with Human pan Cadherin peptide (ab17098) at 1 µg/ml
    Lane 10 : Human heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-rabbit conjugated to Alexafluor 680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa
  • HeLa cells fixed in methanol and stained with ab16505 (2µg/ml). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS. The 2 images show the cells stained with different secondary antibodies, Donkey anti Rabbit FITC (image A) and Donkey anti Rabbit Cy3 (image B). In each case ab16505 stains the plasma membrane.  In image A ab16505 is stained green and in image B ab16505 is stained red. In both images the DNA is stained with DAPI (blue).

  • Immunofluorescence analysis of HeLa cells, staining pan Cadherin (red) with ab16505.

    Cells were fixed with paraformaldehyde, permeabilized in methanol and blocked for 1 hour at room temperature in DPBS containing 2 mg/mL BSA, 1% fish gelatin, 0.1% Triton-X 100 and 5% goat serum. Cells were then incubated for 1 hour at room temperature with the primary antibody diluted in blocking buffer. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Anti-pan Cadherin antibody - Plasma Membrane Marker (ab16505) 使用論文

This product has been referenced in:
  • Kulshrestha A  et al. Vacuolar ATPase 'a2' isoform exhibits distinct cell surface accumulation and modulates matrix metalloproteinase activity in ovarian cancer. Oncotarget 6:3797-810 (2015). IHC-P ; Human . Read more (PubMed: 25686833) »
  • Murphy L  et al. The sheep tetherin paralog oBST2B blocks envelope glycoprotein incorporation into nascent retroviral virions. J Virol 89:535-44 (2015). ICC/IF . Read more (PubMed: 25339764) »

See all 23 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human ovarian cystedenocarcinoma tissues)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: bicarbonate buffer pH 6.0
Permeabilization No
Specification human ovarian cystedenocarcinoma tissues
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 11 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Cell (ovarian carcinoma SKOV-3 cell line)
Specification ovarian carcinoma SKOV-3 cell line
Permeabilization Yes - 0.1% triton X for 5 min at RT
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (Day 9.5 embryo)
Specification Day 9.5 embryo
Permeabilization Yes - 0.1% saponin
Fixative Paraformaldehyde
Username

Dr. Nikhil Mittal

Verified customer

投稿 Sep 23 2013

Thank you for contacting us.

All of our pan-Cadherin antibodies have been tested in IHC-P with human samples and are guaranteed for this application and species.

We have 5 rabbit polyclonals: ab15186, ab45009, ab74635, ab6529, ab1...

Read More

Thank you for your enquiry.

Below is a selection of antibodies that are suitable for detecting the plasma membrane and nuclear envelope. They all work in western blot and I would review the datasheets to confirm which species they are tested...

Read More

Thank you for bringing this topic to our attention.

I have spoken with the lab and they agree that the images do show some stronger staining clustered around the nucleus which does appear to be the golgi. Swiss prot actually states a golgi a...

Read More

DISCOUNT CODE: *** Expiration date: February 28, 2012 I am very pleased to hear you would like to accept our offer and test ab16505 in IHC-Fr. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please subm...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293)
Specification HEK293
Fixative 100% Ethanol
Permeabilization No
Blocking step (agent) for 30 minute(s) · Concentration: 0.1% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 May 21 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (ALL cell lines plasma membrane prep)
Loading amount 10 µg
Specification ALL cell lines plasma membrane prep
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in PBS-
Username

Dr. Mark Holland

Verified customer

投稿 Mar 05 2007

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"