Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Lampreys (Petromyzon marinus) Tissue lysate - whole (Brain and spinal cord protein extracts)
Specification
Brain and spinal cord protein extracts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: ThermoScientific Immunopure peroxidase-conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 17 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
Synuclein in rat brain extracts and recombinant human alpha-, beta-, and gamma- synucleins
Additional data
Additional Notes
nitrocellulose membrane this antibody recognizes monomeric synucleins very well
PubMed ID
PMID: 25273557: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jennifer Morgan
Verified customer
投稿 Feb 26 2015