Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Purified protein (Human primary CD4 T cells)
Specification
Human primary CD4 T cells
Treatment
TCR activated using anti-CD3/CD28 for 3,10 and 30min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 2,5%
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: ~60 kDa Non-specific: ~85 kDa
Exposure
1 minute(s) and 30 second(s)
Negative control
non- activated CD4 T cells
Positive control
CD4 T cells activated with anti-CD3/CD28 for 3,10 and 30 minutes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Marco Craveiro
Verified customer
投稿 Jul 23 2013