製品の概要

  • 製品名Anti-PAM antibody [EPR2643(2)]
    PAM 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2643(2)] to PAM
  • アプリケーション適用あり: WBmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PAM (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: Human fetal heart tissue lysate and PC-3 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
  • 追加情報Is unsuitable for ICC/IF or IHC-P.
  • ターゲット情報

    • 機能Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
    • 配列類似性In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
      In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
      Contains 5 NHL repeats.
    • 細胞内局在Membrane and Secreted. Secreted from secretory granules.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AMD_HUMAN antibody
      • PAL antibody
      • PAM antibody
      • Pancreatic peptidylglycine alpha amidating monooxygenase antibody
      • Peptidyl alpha amidating enzyme antibody
      • Peptidyl alpha hydroxyglycine alpha amidating lyase antibody
      • Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody
      • Peptidylamidoglycolate lyase antibody
      • Peptidylglycine 2 hydroxylase antibody
      • Peptidylglycine alpha amidating monooxygenase antibody
      • Peptidylglycine alpha hydroxylating monooxygenase antibody
      • PHM antibody
      see all

    Anti-PAM antibody [EPR2643(2)] 画像

    • Anti-PAM antibody [EPR2643(2)] (ab109175) at 1/10000 dilution (purified) + Human fetal heart tissue lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 108 kDa
      Observed band size : 108 kDa
      Blocking buffer: 5% NFDM/TBST.
      Dilution buffer: 1%BSA/TBST.
    • All lanes : Anti-PAM antibody [EPR2643(2)] (ab109175) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal heart tissue lysate
      Lane 2 : PC-3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 108 kDa

    Anti-PAM antibody [EPR2643(2)] (ab109175) 使用論文

    ab109175 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109175.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"