The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/12500.
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. May play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process.
Cytoplasm. Endomembrane system. It can exist as a soluble protein as well as a peripheral membrane protein.