Anti-PAK6 抗体 (ab41431)
Key features and details
- Rabbit polyclonal to PAK6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAK6 antibody
PAK6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK6 -
由来種
Rabbit -
特異性
The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein. This reactivity is specifically blocked by pre-incubation with PAK6 N-terminal peptide (immunogen) and is not affected by lambda phosphatase treatment. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.
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特記事項
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41431の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
ターゲット情報
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機能
The activated kinase acts on a variety of targets. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated when activated by CDC42/p21. - Information by UniProt
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参照データベース
- Entrez Gene: 56924 Human
- Entrez Gene: 214230 Mouse
- Entrez Gene: 296078 Rat
- Omim: 608110 Human
- SwissProt: Q9NQU5 Human
- SwissProt: Q3ULB5 Mouse
- Unigene: 513645 Human
- Unigene: 241857 Mouse
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別名
- CDKN1A activated kinase 6 antibody
- EC 2.7.1.37 antibody
- p21 activated protein kinase 6 antibody
see all
画像
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All lanes : Anti-PAK6 antibody (ab41431) at 1/1000 dilution
Lane 1 : Human PAK6 recombinant protein phosphorylated by ERK2
Lane 2 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
Lane 3 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific peptide
Lane 4 : Human PAK6 recombinant protein phosphorylated by ERK2 with PAK6 N-terminal peptide (ab41431 immunogen)
Predicted band size: 75 kDa
Observed band size: 75 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab41431 は論文での使用が確認できていません。