Anti-PAK3 抗体 [EP797Y] (ab40808)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP797Y] to PAK3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PAK3 antibody [EP797Y]
PAK3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP797Y] to PAK3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human PAK3 aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- Fetal brain lysate and human brain glioma.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP797Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40808の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 62 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 62 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. It may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in this gene has been linked to nonsyndromic X-linked mental retardation. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 5063 Human
- Entrez Gene: 18481 Mouse
- Entrez Gene: 29433 Rat
- Omim: 300142 Human
- SwissProt: O75914 Human
- SwissProt: Q61036 Mouse
- SwissProt: Q62829 Rat
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別名
- Beta PAK antibody
- bPAK antibody
- CDKN1A antibody
see all
画像
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Anti-PAK3 antibody [EP797Y] (ab40808) at 1/50000 dilution + fetal brain lysate at 10 µg
Predicted band size: 62 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
ab40808 at a 1:100 dilution staining PAK3 in human brain glioma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab40808 は 3 報の論文で使用されています。
- Gao Z et al. PAK3 promotes the metastasis of hepatocellular carcinoma by regulating EMT process. J Cancer 13:153-161 (2022). PubMed: 34976179
- Lauterborn JC et al. Synaptic actin stabilization protein loss in Down syndrome and Alzheimer disease. Brain Pathol 30:319-331 (2020). PubMed: 31410926
- Zuo Z et al. LncRNA TTN-AS1/miR-134-5p/PAK3 axis regulates the radiosensitivity of human large intestine cancer cells through the P21 pathway and AKT/GSK-3β/β-catenin pathway. Cell Biol Int 44:2284-2292 (2020). PubMed: 32749739