Anti-PAK2 抗体 [EP796Y] (ab76293)
製品の概要
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製品名Anti-PAK2 antibody [EP796Y]
PAK2 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EP796Y] to PAK2
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アプリケーション適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details
適用なし: IP -
種交差性交差種: Mouse, Rat, Human
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免疫原
corresponding to Human PAK2 (N terminal).
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ポジティブ･コントロール
- WB: HeLa cytoplasmic lysate IHC-P; Human breast carcinoma tissue IF/ICC: T47D cell line.
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特記事項
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EP796Y
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab76293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000 - 1/2000. Predicted molecular weight: 58 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP conjugated or polymerized HRP secondary antibody is recommended. Stronger signals have been found using the polymerized HRP secondary. |
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| Flow Cyt | 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
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組織特異性Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
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配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻訳後修飾Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated. -
細胞内局在Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 5062 Human
- Entrez Gene: 224105 Mouse
- Entrez Gene: 29432 Rat
- GenBank: NP_002568.2 Human
- Omim: 605022 Human
- SwissProt: Q13177 Human
- SwissProt: Q8CIN4 Mouse
- SwissProt: Q64303 Rat
see all -
別名
- C-t-PAK2 antibody
- CB422 antibody
- EC 2.7.11.1 antibody
see all
Anti-PAK2 antibody [EP796Y] 画像
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![Western blot - Anti-PAK2 antibody [EP796Y] (ab76293)](http://a.abcam.com/ps/products/76/ab76293/Images/ab76293-260618-ab76293KOWB.jpg)
Western blot - Anti-PAK2 antibody [EP796Y] (ab76293)
Predicted band size : 58 kDaLane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PAK2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human lymph node tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76293 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76293 was shown to specifically react with PAK2 when PAK2 knockout samples were used. Wild-type and PAK2 knockout samples were subjected to SDS-PAGE. ab76293 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Immunocytochemistry/ Immunofluorescence - Anti-PAK2 antibody [EP796Y] (ab76293)](http://a.abcam.com/ps/products/76/ab76293/Images/ab76293-213041-ab76293gr404497edited.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PAK2 antibody [EP796Y] (ab76293)ICC/IF image of ab76293 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76293, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-PAK2 antibody [EP796Y] (ab76293)](http://a.abcam.com/ps/products/76/ab76293/Images/ab76293-3-ab76293FC.jpg)
Flow Cytometry - Anti-PAK2 antibody [EP796Y] (ab76293)Overlay histogram showing HeLa cells stained with ab76293 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76293, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK2 antibody [EP796Y] (ab76293)](http://a.abcam.com/ps/products/76/ab76293/Images/ab76293_IHC_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK2 antibody [EP796Y] (ab76293)ab76293, at a 1/100 dilution, staining PAK2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. -
![Western blot - PAK2 antibody [EP796Y] (ab76293)](http://a.abcam.com/ps/products/76/ab76293/Images/ab76293_WB_1.jpg)
Western blot - PAK2 antibody [EP796Y] (ab76293)Anti-PAK2 antibody [EP796Y] (ab76293) at 1/2000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 58 kDa
Observed band size : 61 kDa (why is the actual band size different from the predicted?)
プロトコール
Anti-PAK2 antibody [EP796Y] (ab76293) 使用論文
This product has been referenced in:
- Jiang N et al. In vivo quantitative phosphoproteomic profiling identifies novel regulators of castration-resistant prostate cancer growth. Oncogene N/A:N/A (2014). Mouse . Read more (PubMed: 25065596) »
- Chen G et al. TGFß receptor I transactivation mediates stretch-induced Pak1 activation and CTGF upregulation in mesangial cells. J Cell Sci 126:3697-712 (2013). Read more (PubMed: 23781022) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"