製品の概要

  • 製品名Anti-PAK1 (phospho T212) antibody
    PAK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PAK1 (phospho T212)
  • 特異性ab75599 detects endogenous levels of PAK1 only when phosphorylated at threonine 212.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).

  • ポジティブ・コントロール
    • Extracts from 293 cells treated with forskolin (40µM, 30min). Human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated when activated by CDC42/p21 and RAC1.
  • 細胞内局在Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Anti-PAK1 (phospho T212) antibody 画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75599, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
  • All lanes : Anti-PAK1 (phospho T212) antibody (ab75599) at 1/500 dilution

    Lane 1 : Extracts from 293 cells (untreated)
    Lane 2 : Extracts from 293 cells treated with forskolin (40µM, 30min),


    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab75599 at 1/100 dilution staining PAK1 (phospho T212) in Hela cells by ICC/IF (MeOH fixation).

Anti-PAK1 (phospho T212) antibody (ab75599) 使用論文

This product has been referenced in:
  • El-Baba C  et al. Thymoquinone-induced conformational changes of PAK1 interrupt prosurvival MEK-ERK signaling in colorectal cancer. Mol Cancer 13:201 (2014). WB ; Human . Read more (PubMed: 25174975) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 50 µg
Specification HCT116
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"