製品の概要

  • 製品名Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody
    PAK1 + PAK2 + PAK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho S141)
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit
  • 免疫原

    Chemically synthesized phosphopeptide derived from a region of human PAK2 that contains serine 141. The sequence is conserved in rat and rabbit.

  • ポジティブ・コントロール
    • Cos cells transiently transfected with PAK 1, PAK 2, or PAK 3, and co transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation.
  • 特記事項


    P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PAK. The final product is generated by affinity chromatography using a PAK 2 derived peptide that is phosphorylated at serine 141.
  • 一次抗体 備考P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 65 kDa.

ターゲット情報

Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody 画像



  • Predicted band size : 65 kDa


    Induction of Activation: Extracts prepared from Cos cells transiently transfected with PAK 1 and V12 Cdc42, and kept in suspension (inactive) or as adherent cultures (active) were resolved by SDSPAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either not treated or treated with lambda phosphatase, blocked with a 5% BSA-TBST buffer overnight at 4°C, and then incubated with 0.50 µg/mL ab5247 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.


  • Predicted band size : 65 kDa


    Peptide Competition: Extracts prepared from Cos cells transiently transfected with PAK 1 (lanes 1-4), PAK 2 (lanes 5-8), or PAK 3 (lanes 9-12) and cotransfected with V12 Cdc42 to induce activation were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5247 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5, 9), the nonphosphopeptide corresponding to the immunogen (2, 6, 10), a generic phosphoserine containing peptide (3, 7, 11), or, the phosphopeptide immunogen (4, 8, 12). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that the ab5247 antibody is highly selective for the phosphorylated form of PAK, and that the antibody can be used to detect an active form of PAK 1 as well as PAK 2 and PAK 3.

Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247) 使用論文

This product has been referenced in:
  • Kuželová K  et al. Group I PAK inhibitor IPA-3 induces cell death and affects cell adhesivity to fibronectin in human hematopoietic cells. PLoS One 9:e92560 (2014). WB ; Human . Read more (PubMed: 24664099) »
  • Awasthi A  et al. Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes. J Exp Med 207:1923-38 (2010). WB ; Mouse . Read more (PubMed: 20733035) »

See all 2 Publications for this product

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