製品の概要

  • 製品名Anti-PAK1 interacting protein 1 antibody
    PAK1 interacting protein 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PAK1 interacting protein 1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-392 of Human PAK1 interacting protein 1

  • ポジティブ・コントロール
    • PAK1 interacting protein 1 transfected 293T cell lysate. HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab67348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
  • 追加情報ICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).

    Note: This product requires purification with Protein A prior to ICC/IF.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性PAK1 interacting protein 1 (PAK1IP1) negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which have been shown to play a positive role in the regulation of signaling pathways involving MAPK8 and RELA. PAK1 exists as an inactive homodimer, which is activated by binding of small GTPases such as CDC42 to an N terminal regulatory domain. PAK1IP1 also binds to the N terminus of PAK1, and inhibits the specific activation of PAK1 by CDC42.
    • 細胞内局在Nuclear, nucleolus.
    • 参照データベース
    • 別名
      • bA421M1.5 antibody
      • FLJ20624 antibody
      • hPIP 1 antibody
      • hPIP1 antibody
      • MAK 11 antibody
      • MAK11 antibody
      • p21 activated protein kinase interacting protein 1 antibody
      • PAK- AND PLC-INTERACTING PROTEIN 1 antibody
      • PAK/PLC interacting protein 1 antibody
      • PAK1 interacting protein antibody
      • PAK1IP1 antibody
      • PIP 1 antibody
      • PIP1 antibody
      • Putative PAK inhibitor Skb15 antibody
      • RP11 421M1.5 antibody
      • WD repeat containing protein 84 antibody
      • WDR 84 antibody
      • WDR84 antibody
      see all

    Anti-PAK1 interacting protein 1 antibody 画像

    • All lanes : Anti-PAK1 interacting protein 1 antibody (ab67348) at 1/500 dilution

      Lane 1 : PAK1 interacting protein 1 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 44 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 44 kDa. We are unsure as to the identity of these extra bands.
    • Immunofluorescent staining of PAK1 interacting protein 1 in HeLa cells using purified ab67348 at 10 ug/ml.

    Anti-PAK1 interacting protein 1 antibody (ab67348) 使用論文

    This product has been referenced in:
    • Yu W  et al. PAK1IP1, a ribosomal stress-induced nucleolar protein, regulates cell proliferation via the p53-MDM2 loop. Nucleic Acids Res 39:2234-48 (2011). WB ; Human . Read more (PubMed: 21097889) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab67348.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"