製品の概要

  • 製品名Anti-PAI1 antibody
    PAI1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PAI1
  • 特異性This antibody is expected to recognise both reported isoforms.
  • アプリケーション適用あり: Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-GFKIDDKGMAPALRH

    , corresponding to Internal sequence amino acids 86-100 of Human PAI1

  • ポジティブ・コントロール
    • Human duodenum, liver and lung lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 1.5 - 3 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 組織特異性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 関連疾患Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 配列類似性Belongs to the serpin family.
  • 翻訳後修飾Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Anti-PAI1 antibody 画像

  • Anti-PAI1 antibody (ab31280) at 0.1 µg/ml + Human Liver lysate at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody.

    See Abreview

  • ab31280 (4µg/ml) staining of paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • ab31280 (1.5ug/ml) used as the reporter with a capture rabbit antibody (2.5ug/ml).

Anti-PAI1 antibody (ab31280) 使用論文

This product has been referenced in:
  • Keates AC  et al. Helicobacter pylori infection stimulates plasminogen activator inhibitor 1 production by gastric epithelial cells. Infect Immun 76:3992-9 (2008). WB ; Human . Read more (PubMed: 18519558) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lung Epithelial cell (Beas2B))
Loading amount 20 µg
Specification Lung Epithelial cell (Beas2B)
Gel Running Conditions Reduced Denaturing (gel 8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Jul 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Treatment Homogenised in 1% SDS lysis buffer
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Dr. Rachel Barker

Verified customer

投稿 Dec 08 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (MDA-MB-231 Breast Cancer Cells, grown in mouse bon)
Specification MDA-MB-231 Breast Cancer Cells, grown in mouse bon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate EDTA
Permeabilization No
Blocking step Casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
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Abcam user community

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投稿 Jul 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Bone Marrow Stromal Cells (HS-5))
Loading amount 5 µg
Specification Human Bone Marrow Stromal Cells (HS-5)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step 1x Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Verified customer

投稿 Jun 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"