製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PAI1
  • アプリケーション
    適用あり: IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recombinant full length PAI1 protein (Mouse).

  • ポジティブ・コントロール
    • Paraffin sections of lung and carotid.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 18276785
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 1 - 10 µg/ml.

ターゲット情報

  • 機能
    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 組織特異性
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 関連疾患
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 配列類似性
    Belongs to the serpin family.
  • 翻訳後修飾
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Anti-PAI1 antibody 画像

  • ab28207 staining PAI1 in E18.5 mouse tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Subsequent to deparaffinization, the sections were hydrated, heated in 10mM citrate buffer (pH 6.0) and treated with 1% H2O2 in methanol for 10 min and blocked with 10% of normal serum. The primary antibody was incubated with sample at 4°C overnight.

Anti-PAI1 antibody (ab28207) 使用論文

This product has been referenced in:
  • Magnussen SN  et al. Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - ß1 (TGF-ß1) and potential effects on migration and invasion. BMC Cancer 17:350 (2017). Read more (PubMed: 28526008) »
  • Swaminathan SS  et al. TGF-ß2-mediated ocular hypertension is attenuated in SPARC-null mice. Invest Ophthalmol Vis Sci 55:4084-97 (2014). IHC . Read more (PubMed: 24906856) »

See all 10 Publications for this product

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Thanks for your enquiry and for providing the protocol and data.

To our knowledge, this antibody has not previously been tested for reactivity to human PAI-1. It may be there is no reactivity to the human protein. Do you happen to have any mo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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