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Our Abpromise guarantee covers the use of ab28207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 18276785|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.|
|IHC-P||Use a concentration of 1 - 10 µg/ml.|
ab28207 staining PAI1 in E18.5 mouse tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Subsequent to deparaffinization, the sections were hydrated, heated in 10mM citrate buffer (pH 6.0) and treated with 1% H2O2 in methanol for 10 min and blocked with 10% of normal serum. The primary antibody was incubated with sample at 4°C overnight.