製品の概要

  • 製品名Anti-PAI1 antibody
    PAI1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PAI1
  • 特異性Ab20562 recognizes Human PAI-1 both alone and in complex under reducing and native conditions.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant full length protein (Human PAI1).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab20562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/50000.
WB 1/5000. Predicted molecular weight: 45 kDa. Dilution optimised using Chromogenic detection

ターゲット情報

  • 機能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 組織特異性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 関連疾患Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 配列類似性Belongs to the serpin family.
  • 翻訳後修飾Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Anti-PAI1 antibody (ab20562) 使用論文

This product has been referenced in:
  • Ahmed Z  et al. Decorin blocks scarring and cystic cavitation in acute and induces scar dissolution in chronic spinal cord wounds. Neurobiol Dis 64:163-76 (2014). Read more (PubMed: 24384090) »
  • Wang L  et al. uPA binding to PAI-1 induces corneal myofibroblast differentiation on vitronectin. Invest Ophthalmol Vis Sci 53:4765-75 (2012). WB . Read more (PubMed: 22700714) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (jurkat cells)
Gel Running Conditions Reduced Denaturing
Loading amount 100000 cells
Specification jurkat cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jul 31 2012

Thank you for contacting us.

Most likely the lower band is for total PAI1 and the upper band is for glycosylated PAI1. This protein gets glycosylated at at least 3 locations according to Swiss Prot, so this post translational modification can ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (carcinoma)
Gel Running Conditions Non-reduced Denaturing
Loading amount 15 µg
Specification carcinoma
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Nov 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"