製品の概要

  • 製品名
    Anti-PAI1 antibody [1D5]
    PAI1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1D5] to PAI1
  • アプリケーション
    適用あり: WB, ELISA, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Purified recombinant fragment of Human PAI1 expressed in E. Coli.

  • ポジティブ・コントロール
    • Recombinant Human PAI1 protein (aa194-316); PAI1 (aa194-316)-hIgGFc transfected HEK293 cell lysate; Human lung cancer and kidney cancer tissues; NIH 3T3 cells.
  • 特記事項

    This product was changed from ascites to supernatant. Lot no’s high than GR201079-27 are from Tissue Culture Supernatant

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 45 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/200 - 1/1000.

ターゲット情報

  • 機能
    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 組織特異性
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 関連疾患
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 配列類似性
    Belongs to the serpin family.
  • 翻訳後修飾
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

画像

  • Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution + Human PAI1 recombinant protein (amino acids 194-316)

    Predicted band size : 45 kDa
  • All lanes : Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PAI1-hIgGFc transfected HEK293 cell lysate (aa194-316)


    Predicted band size : 45 kDa
  • ELISA using ab125687.
  • ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human lung cancer tissue by Immunohistochemistry followed by DAB staining.
  • ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human kidney cancer tissue by Immunohistochemistry followed by DAB staining.
  • ab125687 at 1/200 dilution staining PAI1 in NIH 3T3 cells by Flow Cytometry (green), negative control (red).

参考文献

This product has been referenced in:
  • Li Y  et al. LPS induces HUVEC angiogenesis in vitro through miR-146a-mediated TGF-ß1 inhibition. Am J Transl Res 9:591-600 (2017). WB ; Human . Read more (PubMed: 28337286) »
  • Liang Q  et al. Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice. Iran J Basic Med Sci 19:43-8 (2016). WB ; Mouse . Read more (PubMed: 27096063) »

See all 3 Publications for this product

レビューと Q&A

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The price for antibody ab125687 is €390 plus €39 shipping charges to Switzerland.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or ...

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Thank you for your reply.

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Merci de votre question et votre intérêt pour nos produits.

L'anticorps secondaire ab98689 n'est pas couplé à la biotine - c'est juste l'anticorps secondaire non-conjugué. Les anticorps pré-adsorb&eacu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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