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Synthetic phosphopeptide derived from the region of human PAG3 that contains tyrosine 724.
Our Abpromise guarantee covers the use of ab18031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 112 kDa).|
Lane 1: wild-type PAG3 alone
Lane 2: wild-type PAG3 cotransfected with mouse PyK2
Lane 3: wild-type PAG3 cotransfected with mouse PyK2. Antibody blocked with the non phosphopeptde corresponding to the immunogen
Lane 4: wild-type PAG3 cotransfected with mouse PyK2. Antibody blocked with the generic phosphotyrosine corresponding to the immunogen
Lane 5: wild-type PAG3 cotransfected with mouse PyK2. Antibody blocked with the generic phosphopeptide immunogen
Lane 6: mutant [Y724/Y763] PAG3 cotransfected with Pyk2
10-30 micrograms of cell lysate can be loaded when using similar lysates with this antibody. Samples were run using SDS-PAGE on a 8% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with PAG3 antibody Ab18031 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with blocking peptides. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate in 3% BSA-TBST buffer, and bands were detected using the Pierce SuperSignal method.
Only the phospho peptide immunogen blocks the antibody, confirming its specificity for the phospho epitope tyrosine 724.
ab18031 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"