製品の概要

  • 製品名Anti-PAG3 (phospho Y724) antibody
    PAG3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PAG3 (phospho Y724)
  • 特異性Lysates prepared from COS cells overexpressing human wild-type PAG3 and cotransfected with mouse Pyk2 were immunoblotted in the presence of non-phosphopeptide corresponding to the immunogen, a generic phosphotyrosine-containing peptide or the phosphopeptide immunogen. The data show that only the peptide corresponding to phospho Y724 PAG3 blocks the signal, and the signal is absent when mutant PAG3 extracts are used, demonstrating the specificity of the antibody.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    Reacts with human PAG3 Predicted to react with mouse PAG3 due to 92% sequence homology
  • 免疫原

    Synthetic phosphopeptide derived from the region of human PAG3 that contains tyrosine 724.

  • ポジティブ・コントロール
    • COS cells overexpressing human wild type PAG3, cotransfected with mouse Pyk2.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and
    Ca2+), 1mg/ml BSA. pH 7.3.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated PAG3 protein. The final product is generated by affinity chromatography using a PAG3 derived peptide that is phosphorylated at tyrosine 724.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab18031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 112 kDa).

ターゲット情報

  • 機能Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.
  • 組織特異性Detected in heart, brain, placenta, kidney, monocytes and pancreas.
  • 配列類似性Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • ドメインThe conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.
  • 翻訳後修飾Phosphorylated on tyrosine residues by SRC and PTK2B.
  • 細胞内局在Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Colocalizes with F-actin and ARF6 in phagocytic cups.
  • Information by UniProt
  • 別名
    • AMAP 2 antibody
    • AMAP2 antibody
    • ANK repeat and PH domain-containing protein 2 antibody
    • Arf-GAP with SH3 domain antibody
    • ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 antibody
    • ASAP2 antibody
    • ASAP2_HUMAN antibody
    • centaurin, beta 3 antibody
    • CENTB3 antibody
    • DDEF 2 antibody
    • DDEF2 antibody
    • Development and differentiation enhancing factor 2 antibody
    • Development and differentiation-enhancing factor 2 antibody
    • FLJ42910 antibody
    • KIAA0400 antibody
    • PAG 3 antibody
    • PAG3 antibody
    • Pap alpha antibody
    • PAP antibody
    • Paxillin associated protein with ARFGAP activity 3 antibody
    • Paxillin-associated protein with ARF GAP activity 3 antibody
    • Pyk2 C terminus associated protein antibody
    • Pyk2 C-terminus-associated protein antibody
    • SHAG 1 antibody
    • SHAG1 antibody
    see all

Anti-PAG3 (phospho Y724) antibody 画像



  • Predicted band size : 112 kDa


    Western blot using Ab18031 on COS cell lysate overexpressing human PAG3.

    Lane 1: wild-type PAG3 alone
    Lane 2: wild-type PAG3 cotransfected with mouse PyK2
    Lane 3: wild-type PAG3 cotransfected with mouse PyK2. Antibody blocked with the non phosphopeptde corresponding to the immunogen
    Lane 4: wild-type PAG3 cotransfected with mouse PyK2. Antibody blocked with the generic phosphotyrosine corresponding to the immunogen
    Lane 5: wild-type PAG3 cotransfected with mouse PyK2. Antibody blocked with the generic phosphopeptide immunogen
    Lane 6: mutant [Y724/Y763] PAG3 cotransfected with Pyk2

    10-30 micrograms of cell lysate can be loaded when using similar lysates with this antibody. Samples were run using SDS-PAGE on a 8% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with PAG3 antibody Ab18031 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with blocking peptides. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate in 3% BSA-TBST buffer, and bands were detected using the Pierce SuperSignal method.

    Only the phospho peptide immunogen blocks the antibody, confirming its specificity for the phospho epitope tyrosine 724.

Anti-PAG3 (phospho Y724) antibody (ab18031) 使用論文

ab18031 has not yet been referenced specifically in any publications.

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