製品の概要

  • 製品名Anti-PAG antibody [MEM-255]
    PAG 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-255] to PAG
  • 特異性This antibody is specific for CSK Binding Protein
  • アプリケーション適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human CSK Binding Protein.

  • ポジティブ・コントロール
    • RAMOS and Jurkat cells
  • 特記事項Csk-Binding Protein is also known as PAG (Protein Associated with Glycosphingolipid-enriched microdomains).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by protein A-affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名MEM-255
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at an assay dependant dilution. Use a lysis buffer made with Tris HCl (pH 8.1) and 1%laurylmaltoside (with common protease inhibitors). Use under non-reducing conditions.
    Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. The reason is, that PAG has a pI value of about 4.6 and binds poorely SDS and therefore it had decreased mobility on SDS gels.

    Is unsuitable for IP (use ab4206 or ab14989).

    May be unsuitable for ICC-IF due to unsuccesful results in submitted Abreview. (Please see Abreview comments)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
    • 組織特異性Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
    • 翻訳後修飾Palmitoylated.
      Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
    • 細胞内局在Cell membrane. Present in lipid rafts.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CBP antibody
      • Csk binding protein antibody
      • Csk-binding protein antibody
      • FLJ37858 antibody
      • MGC138364 antibody
      • PAG 1 antibody
      • PAG antibody
      • PAG1 antibody
      • PAG1_HUMAN antibody
      • Phosphoprotein associated with glycosphingolipid enriched microdomains 1 antibody
      • Phosphoprotein associated with glycosphingolipid enriched microdomains antibody
      • Phosphoprotein associated with glycosphingolipid microdomains 1 antibody
      • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 antibody
      • Protein Associated with Glycosphingolipid Enriched Microdomains antibody
      • Transmembrane adapter protein PAG antibody
      • Transmembrane phosphoprotein Cbp antibody
      see all

    Anti-PAG antibody [MEM-255] (ab9527) 使用論文

    This product has been referenced in:
    • Mutch CM  et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). Read more (PubMed: 17090619) »
    • Brdickova N  et al. Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett 507:133-6 (2001). Read more (PubMed: 11684085) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa cells)
    Specification HeLa cells
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    投稿 Mar 22 2007

    Thank you for your enquiry. CSK Binding Protein antibody Ref:ab9527 [MEM-255] should stain blots strongly in dilutions 1:500 - 1:1000 but optimum dilution should be done for a particular system of a researcher. We did not measure protein load, it alway...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"