Anti-RPA32/RPA2 抗体 [MA34] (ab111161)
Key features and details
- Mouse monoclonal [MA34] to RPA32/RPA2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgM
製品の概要
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製品名
Anti-RPA32/RPA2 antibody [MA34]
RPA32/RPA2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MA34] to RPA32/RPA2 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99.85% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MA34 -
アイソタイプ
IgM -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111161の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
1/10 - 1/100.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/10 - 1/100. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
翻訳後修飾
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
細胞内局在
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 6118 Human
- Entrez Gene: 59102 Rat
- Omim: 179836 Human
- SwissProt: P15927 Human
- SwissProt: Q63528 Rat
- Unigene: 703070 Human
- Unigene: 79411 Human
- Unigene: 40389 Rat
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別名
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
画像
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Anti-RPA32/RPA2 antibody [MA34] (ab111161) at 1/500 dilution + Purified RPA32/RPA2 protein (human)
Predicted band size: 55 kDa -
Immunofluorescent analysis of RPA32/RPA2 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA32/RPA2 monoclonal antibody (ab111161) at a dilution of 1/200 overnight at 4°C and incubated with a DyLight-488 conjugated secondary antibody. RPA32/RPA2 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunofluorescent analysis of RPA32/RPA2 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA32/RPA2 monoclonal antibody (ab111161) at a dilution of 1/200 overnight at 4°C and incubated with a DyLight-488 conjugated secondary antibody. RPA32/RPA2 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunofluorescent analysis of RPA32/RPA2 in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA32/RPA2 monoclonal antibody (ab111161) at a dilution of 1/200 overnight at 4°C and incubated with a DyLight-488 conjugated secondary antibody. RPA32/RPA2 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a RPA32/RPA2 monoclonal antibody (ab111161) at a dilution of 1/20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a RPA32/RPA2 monoclonal antibody (ab111161) at a dilution of 1/20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a RPA32/RPA2 monoclonal antibody (ab111161) at a dilution of 1/20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab111161 は論文での使用が確認できていません。