Anti-PAF1/PD2 抗体 (ab137519)
Key features and details
- Rabbit polyclonal to PAF1/PD2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAF1/PD2 antibody
PAF1/PD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAF1/PD2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human PAF1/PD2 aa 232-481.
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ポジティブ・コントロール
- WB: Wild-type HEK-293T, A549, Daudi, A431 and H1299 whole cell lysates. IHC-P: Cal27 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137519の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using
10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ターゲット情報
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機能
The mammalian PAF1 complex is a multifunctional complex. The PAF1 complex interacts with RNA polymerase II and may be involved in both initiation and elongation, and in histone methylation and RNA processing. Has oncogenic activity in vivo and in vitro. -
配列類似性
Belongs to the PAF1 family. -
細胞内局在
Nucleus. Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 528744 Cow
- Entrez Gene: 54623 Human
- Entrez Gene: 54624 Mouse
- Entrez Gene: 361531 Rat
- Omim: 610506 Human
- SwissProt: Q2KJ14 Cow
- SwissProt: Q8N7H5 Human
- SwissProt: Q8K2T8 Mouse
see all -
別名
- F23149 1 antibody
- FLJ11123 antibody
- hPAF1 antibody
see all
画像
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All lanes : Anti-PAF1/PD2 antibody (ab137519) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PAF1 CRISPR-Cas9 Edited HEK-293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab137519 observed at 80 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab137519 was shown to react with PAF1/PD2 in wild-type HEK-293T cells in Western blot with loss of signal observed at the expected molecular weight in PAF1 CRISPR-Cas9 edited cell line. The band observed at the higher molecular weight in the CRISPR-Cas9 edited sample is likely to represent PAF1 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. Wild-type HEK-293T and PAF1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab137519 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-PAF1/PD2 antibody (ab137519) at 1/5000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
7.5 % SDS PAGE -
Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labelling PAF1/PD2 using ab137519 at 1/500.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab137519 は 5 報の論文で使用されています。
- Chen Z et al. Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling. Nat Commun 12:6318 (2021). PubMed: 34732721
- Zheng JJ et al. Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription. Radiat Oncol 15:131 (2020). PubMed: 32471508
- Zhang M et al. A peptide encoded by circular form of LINC-PINT suppresses oncogenic transcriptional elongation in glioblastoma. Nat Commun 9:4475 (2018). PubMed: 30367041
- Herr P et al. A genome-wide IR-induced RAD51 foci RNAi screen identifies CDC73 involved in chromatin remodeling for DNA repair. Cell Discov 1:15034 (2015). Human . PubMed: 27462432
- Chen FX et al. PAF1, a Molecular Regulator of Promoter-Proximal Pausing by RNA Polymerase II. Cell 162:1003-15 (2015). PubMed: 26279188