製品の概要

  • 製品名Anti-PAF Receptor antibody
    PAF Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PAF Receptor
  • アプリケーション適用あり: WB, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Rhesus monkey
  • 免疫原

    Synthetic peptide:

    MEPHDSSHMDSEFRYTL

    conjugated to KLH, corresponding to N terminal amino acids 1-17 of Human PAF Receptor (Peptide available as ab107706.)

  • ポジティブ・コントロール
    • A549 membrane fraction. U937 and Raji cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab104162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200. Predicted molecular weight: 39 kDa.Can be blocked with PAF Receptor peptide (ab107706).
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

Anti-PAF Receptor antibody 画像

  • All lanes : Anti-PAF Receptor antibody (ab104162) at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : A549 membrane fraction at 30 µg

    Secondary
    Anti-rabbit IgG (HRP) conjugate at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 39 kDa
  • PAF Receptor was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to PAF Receptor and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104162.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 39kDa; PAF Receptor

Anti-PAF Receptor antibody (ab104162) 使用論文

This product has been referenced in:
  • Yu Y  et al. The expression of platelet-activating factor receptor modulates the cisplatin sensitivity of ovarian cancer cells: a novel target for combination therapy. Br J Cancer N/A:N/A (2014). WB . Read more (PubMed: 24921917) »
  • Yuan D  et al. Enhanced permeability responses to inflammation in streptozotocin-induced diabetic rat venules: Rho-mediated alterations of actin cytoskeleton and VE-cadherin. Am J Physiol Heart Circ Physiol 307:H44-53 (2014). Read more (PubMed: 24778164) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"