製品の概要

  • 製品名Anti-PADPR antibody
    PADPR 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to PADPR
  • 特異性This antibody recognizes PADPR synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes, including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. It does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. It does cross-react to bovine serum albumin due to its use as a carrier for the immunogen.
  • アプリケーション適用あり: ICC/IF, IHC-Fr, WB, ELISA, IHC-Pmore details
  • 種交差性
    Predicted to react with a wide range of species due to sequence homology.
  • 免疫原

    Purified PADPR mixed with methylated bovine serum albumin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
IHC-Fr Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 1 kDa.
ELISA Use at an assay dependent dilution. It is recommended to start with a 1/100 dilution.
IHC-P Use at an assay dependent dilution.

ターゲット情報

  • 関連性PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).
  • 別名
    • Poly ADP ribose antibody

Anti-PADPR antibody 画像

  • PADPR staining of untreated rat liver, with ab14460 at a concentration of 20µg/ml.



  • Predicted band size : 1 kDa


    Western blot analysis of PADPR. Lane 1: control HL60 cells (75,000 cells). Lane 2: cells automodified with PARP1. Note the apparent shift in molecular weight PARP1 from 116kDa to a broad range >250kDa due to various extents of PADPR automodification. ab14460 was used at a concentration of 10 µg/ml.
  • PADPR staining of livers from rats injected with diethylnitrosamine (200 mg/kg). The livers were removed and rapidly processed 10 hr later, at peak polymer induction. ab14460 was used at a concentration of 20µg/ml
  • ab14460 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C.  A Cy5® conjugated donkey anti-chicken antibody diluted 1/300 was used as the secondary.

    See Abreview

Anti-PADPR antibody (ab14460) 使用論文

This product has been referenced in:
  • Kheradpezhouh E  et al. TRPM2 channels mediate acetaminophen-induced liver damage. Proc Natl Acad Sci U S A 111:3176-81 (2014). ICC/IF ; Mouse . Read more (PubMed: 24569808) »
  • Masaoka A  et al. HMGN1 Protein Regulates Poly(ADP-ribose) Polymerase-1 (PARP-1) Self-PARylation in Mouse Fibroblasts. J Biol Chem 287:27648-58 (2012). ICC/IF ; Mouse . Read more (PubMed: 22736760) »

See all 3 Publications for this product

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Thank you for your message. I am very pleased to hear you would like to accept our offer and test ab14459 and ab14460 in IP.

ab14459
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Expiration date: 4th Feb 2013

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Thank you for your message. i hope you enjoyed the EMBO conference.

I am sorry to confirm that to our knowledge, ab14459 and ab14460 have not been tested in IP. They have been tested in western blotting.

Therefore, I can offer a d...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Alexander Rapp

Verified customer

投稿 Apr 18 2008

It is recommended to start at a 1:100 dilution. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"