アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Purified PADPR mixed with methylated bovine serum albumin.
Our Abpromise guarantee covers the use of ab14460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 1 kDa.|
|ELISA||Use at an assay dependent dilution. It is recommended to start with a 1/100 dilution.|
|IHC-P||Use at an assay dependent dilution.|
PADPR staining of untreated rat liver, with ab14460 at a concentration of 20
ab14460 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy5® conjugated donkey anti-chicken antibody diluted 1/300 was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"