This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

製品の概要

製品の特性

アプリケーション

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

アプリケーション Abreviews 特記事項
WB
  • 追加情報IF/ICC: see image below

    This antibody gave a positive result in ELISA against the immunizing peptide (ab17094).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Catalyzes the deimination of arginine residues of proteins. May be involved in cytoskeletal reorganization in the egg and early embryo.
    • 組織特異性Ovary, testis and peripheral blood leukocytes.
    • 配列類似性Belongs to the protein arginine deiminase family.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • hPADVI antibody
      • PADI6 antibody
      • PADI6_HUMAN antibody
      • peptidyl arginine deiminase like protein antibody
      • peptidyl arginine deiminase type VI antibody
      • Peptidylarginine deiminase type 6 antibody
      • Peptidylarginine deiminase type VI antibody
      • Peptidylarginine deiminase VI antibody
      • protein arginine deiminase type 6 antibody
      • Protein arginine deiminase type VI antibody
      • Protein-arginine deiminase type VI antibody
      • Protein-arginine deiminase type-6 antibody
      see all

    Anti-PADI6 antibody 画像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-PADI6 antibody (ab16480) at 1 µg/ml

      Lane 1 : Human ovary lysate
      Lane 2 : Human testes lysate
      Lane 3 : Human ovary lysate with Human PADI6 peptide (ab17094) at 1 µg/ml
      Lane 4 : Human testes lysate with Human PADI6 peptide (ab17094) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
      Developed using the ECL technique
    • ICC/IF image of ab16480 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16480, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 100% methanol fixed cells at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

    Anti-PADI6 antibody (ab16480) 使用論文

    ab16480 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa #Jurkat #HL-60)
    Loading amount 30 µg
    Specification HeLa #Jurkat #HL-60
    Gel Running Conditions Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    投稿 Oct 28 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"