Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).
IF/ICC: see image below
This antibody gave a positive result in ELISA against the immunizing peptide (ab17094).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Catalyzes the deimination of arginine residues of proteins. May be involved in cytoskeletal reorganization in the egg and early embryo.
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
Western blot - PADI6 antibody (ab16480)
All lanes : Anti-PADI6 antibody (ab16480) at 1 µg/ml
Lane 1 : Human ovary lysate
Lane 2 : Human testes lysate
Lane 3 : Human ovary lysate
with Human PADI6 peptide (ab17094) at 1 µg/ml Lane 4 : Human testes lysate
with Human PADI6 peptide (ab17094) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution Developed using the ECL technique
ICC/IF image of ab16480 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16480, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 100% methanol fixed cells at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.