製品の概要

  • 製品名Anti-PADI4 / PAD4 antibody
    PADI4 / PAD4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PADI4 / PAD4
  • アプリケーション適用あり: WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) from the C-terminal region conjugated to KLH

  • ポジティブ・コントロール
    • 293 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.

ターゲット情報

  • 機能Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • 組織特異性Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
  • 関連疾患Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
  • 配列類似性Belongs to the protein arginine deiminase family.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 3.5.3.15 antibody
    • HL 60 PAD antibody
    • HL-60 PAD antibody
    • PAD 4 antibody
    • PAD antibody
    • PADI 4 antibody
    • PADI 5 antibody
    • PADI H protein antibody
    • Padi4 antibody
    • PADI4_HUMAN antibody
    • PADI5 antibody
    • PDI 4 antibody
    • PDI 5 antibody
    • PDI4 antibody
    • PDI5 antibody
    • Peptidyl arginine deiminase type IV antibody
    • Peptidyl arginine deiminase type V antibody
    • Peptidylarginine deiminase IV antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 4 antibody
    • Protein arginine deiminase type IV antibody
    • Protein-arginine deiminase type IV antibody
    • Protein-arginine deiminase type-4 antibody
    see all

Anti-PADI4 / PAD4 antibody 画像

  • ICC/IF image of ab59965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59965 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PADI4 / PAD4 antibody (ab59965) at 1/100 dilution + 293 cell lysate

    Predicted band size : 74 kDa
    Observed band size : 74 kDa

Anti-PADI4 / PAD4 antibody (ab59965) 使用論文

ab59965 has not yet been referenced specifically in any publications.

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