製品の概要

  • 製品名Anti-PADI4 / PAD4 antibody
    PADI4 / PAD4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PADI4 / PAD4
  • アプリケーション適用あり: IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to amino acids 1-15 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue

  • ポジティブ・コントロール
    • Extracts of DMSO-treated HL60 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution. 2-4 µl of the antibody immunoprecipitatesPADI4 from DMSO-treated HL60 cells
WB 1/1000 - 1/2000. Predicted molecular weight: 74 kDa.

ターゲット情報

  • 機能Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • 組織特異性Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
  • 関連疾患Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
  • 配列類似性Belongs to the protein arginine deiminase family.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 3.5.3.15 antibody
    • HL 60 PAD antibody
    • HL-60 PAD antibody
    • PAD 4 antibody
    • PAD antibody
    • PADI 4 antibody
    • PADI 5 antibody
    • PADI H protein antibody
    • Padi4 antibody
    • PADI4_HUMAN antibody
    • PADI5 antibody
    • PDI 4 antibody
    • PDI 5 antibody
    • PDI4 antibody
    • PDI5 antibody
    • Peptidyl arginine deiminase type IV antibody
    • Peptidyl arginine deiminase type V antibody
    • Peptidylarginine deiminase IV antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 4 antibody
    • Protein arginine deiminase type IV antibody
    • Protein-arginine deiminase type IV antibody
    • Protein-arginine deiminase type-4 antibody
    see all

Anti-PADI4 / PAD4 antibody 画像

  • All lanes : Anti-PADI4 / PAD4 antibody (ab50332) at 1/1000 dilution

    Lane 1 : 293-T cells
    Lane 2 : 293-T cells transfected with PADI4 cDNA
    Lane 3 : HL60 cells
    Lane 4 : HL60 cells induced with 1.25% DMSO for three days

    Secondary
    Goat Anti-Rabbit IgG

    Predicted band size : 74 kDa
    Observed band size : ~62 kDa (why is the actual band size different from the predicted?)

Anti-PADI4 / PAD4 antibody (ab50332) 使用論文

This product has been referenced in:
  • Sharma P  et al. Citrullination of Histone H3 Interferes with HP1-Mediated Transcriptional Repression. PLoS Genet 8:e1002934 (2012). WB . Read more (PubMed: 23028349) »
  • Tanikawa C  et al. Regulation of protein Citrullination through p53/PADI4 network in DNA damage response. Cancer Res 69:8761-9 (2009). WB ; Human . Read more (PubMed: 19843866) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. The exact protocols are as below: 1. DMSO was added to a concentration of 1.25% to normal HL60 media. This would be the equivalent to adding 125ul DMSO to 10ml media. No additional buffers were required. 2. The HL...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"