製品の概要

  • 製品名Anti-PADI4 / PAD4 antibody
    PADI4 / PAD4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PADI4 / PAD4
  • 特異性This antibody reacts specifically with the 74 kDa PADI4 / PAD4 protein. It shows no cross reactivity with the other isoforms.
  • アプリケーション適用あり: WB, IP, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    A 15 amino acid synthetic peptide derived from the C-terminal domain of human PADI4/ PAD4.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/2000. Predicted molecular weight: 74 kDa.Can be blocked with PADI4 / PAD4 peptide (ab156172).
IP 1/20 - 1/100.
ELISA 1/1000 - 1/5000.
ICC/IF 1/200.

ターゲット情報

  • 機能Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • 組織特異性Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
  • 関連疾患Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
  • 配列類似性Belongs to the protein arginine deiminase family.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 3.5.3.15 antibody
    • HL 60 PAD antibody
    • HL-60 PAD antibody
    • PAD 4 antibody
    • PAD antibody
    • PADI 4 antibody
    • PADI 5 antibody
    • PADI H protein antibody
    • Padi4 antibody
    • PADI4_HUMAN antibody
    • PADI5 antibody
    • PDI 4 antibody
    • PDI 5 antibody
    • PDI4 antibody
    • PDI5 antibody
    • Peptidyl arginine deiminase type IV antibody
    • Peptidyl arginine deiminase type V antibody
    • Peptidylarginine deiminase IV antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 4 antibody
    • Protein arginine deiminase type IV antibody
    • Protein-arginine deiminase type IV antibody
    • Protein-arginine deiminase type-4 antibody
    see all

Anti-PADI4 / PAD4 antibody 画像

  • ICC/IF image of ab50247 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50247, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PADI4 / PAD4 antibody (ab50247) 使用論文

This product has been referenced in:
  • Sylvestersen KB  et al. Proteomic analysis of arginine methylation sites in human cells reveals dynamic regulation during transcriptional arrest. Mol Cell Proteomics 13:2072-88 (2014). Read more (PubMed: 24563534) »

See 1 Publication for this product

Product Wall



The PAD4 protein is predicted to run around 74 kDa based on the molecular weight of the amino acid sequence, however full length PAD4 has been shown to migrate around 67 kDa in SDS-PAGE:

http://rheumatology.oxfordjournals.org/conten...

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I am writing to inform you that the blocking peptide for ab50247 has now been added to our catalog. The product id is PADI4 / PAD4 peptide (ab156172). The datasheet for this product may be found at: http://www.abcam.com/index.html?datasheet=156172

Thank you for contacting us.

The exact concentration is necessary when you would like to use Isotype control in IHC or Immunoprecipitation other than that it is OK.

Please note that we can only provide exact concentration for ab128089...

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Thank you for your reply.

There are a few variations that may work:

1 - Depending on the blocking agent you are using, you could try using 5% BSA, as this may help reduce the background levels that you are seeing.

2 - If y...

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Thank you for contacting Abcam.

If you could provide me with the protocol that you are using, I would be happy to see if there are any protocol suggestions I can make that may help resolve some of the issues we are having.

Also we h...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Lung)
Specification Lung
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
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投稿 Apr 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"