Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PACSIN2 (green) with ab37615 at 1/100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/400) was used as the secondary antibody. Cytoplasmic actin was counterstained using a DyLight Fluor® 554-conjugated Phalloidin (red).
ICC/IF image of ab37615 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37615, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab37615 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.