製品の概要

  • 製品名
    Anti-PACSIN2 antibody
  • 製品の詳細
    Rabbit polyclonal to PACSIN2
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human PACSIN2 aa 342-371 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • WB: Mouse brain and heart tissue lysates. T-47D, Daudi and HL-60 cell lysates. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • 特記事項(精製)
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    May play a role in vesicle formation and transport.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the PACSIN family.
    Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾
    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
  • 細胞内局在
    Cytoplasmic vesicle. Vesicle-like cytoplasmic distribution.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cytoplasmic phosphoprotein PACSIN2 antibody
    • OTTHUMP00000198281 antibody
    • OTTHUMP00000198282 antibody
    • OTTHUMP00000198283 antibody
    • OTTHUMP00000198284 antibody
    • PACN2_HUMAN antibody
    • Pacsin2 antibody
    • protein kinase C and casein kinase substrate in neurons 2 antibody
    • Protein kinase C and casein kinase substrate in neurons protein 2 antibody
    • SDPII antibody
    • Syndapin II antibody
    see all

画像

  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the primary antibody at 1/50, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
  • All lanes : Anti-PACSIN2 antibody (ab37615)

    Lane 1 : HL-60 cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse heart lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 56 kDa
  • All lanes : Anti-PACSIN2 antibody (ab37615) at 1/100 dilution

    Lane 1 : T-47D cell lysate
    Lane 2 : Mouse brain tissue lysate


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PACSIN2 (green) with ab37615 at 1/100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/400) was used as the secondary antibody. Cytoplasmic actin was counterstained using a DyLight Fluor® 554-conjugated Phalloidin (red).

  • ICC/IF image of ab37615 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37615, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab37615 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Begonja AJ  et al. FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets. Blood 126:80-8 (2015). Read more (PubMed: 25838348) »

See 1 Publication for this product

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