製品の概要

  • 製品名Anti-PACAP receptor antibody
    PACAP receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PACAP receptor
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant fragment: MHSDCIFKKE QAMCLEKIQR ANELMGFNDS SPGCPGMWDN ITCWKPAHVG EMVLVSCPEL FRIFNPDQVW ETETIGESDF GDSNSLDLSD MGVVSRNCTE , corresponding to amino acids 21-121 of Human PACAP receptor

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.
  • 組織特異性Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta.
  • 配列類似性Belongs to the G-protein coupled receptor 2 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADCYAP1 R1 antibody
    • ADCYAP1R 1 antibody
    • ADCYAP1R1 antibody
    • Adenylate cyclase activating polypeptide 1 (pituitary) receptor type 1 antibody
    • Adenylate cyclase activating polypeptide 1 (pituitary) receptor type I antibody
    • PAC 1 antibody
    • PAC1 antibody
    • PAC1R antibody
    • PACAP receptor 1 antibody
    • PACAP type I receptor antibody
    • PACAP-R-1 antibody
    • PACAP-R1 antibody
    • PACAP1 R antibody
    • PACAPR antibody
    • PACAPRI antibody
    • PACR_HUMAN antibody
    • Pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop antibody
    • Pituitary adenylate cyclase activating polypeptide receptor antibody
    • Pituitary adenylate cyclase activating polypeptide type I receptor precursor antibody
    • Pituitary adenylate cyclase-activating polypeptide 1, receptor for antibody
    • Pituitary adenylate cyclase-activating polypeptide type I receptor antibody
    • pvr1 antibody
    see all

Anti-PACAP receptor antibody 画像



  • Predicted band size : 53 kDa
    PACAP receptor antibody (ab54980) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab54980 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54890, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PACAP receptor antibody (ab54980) 使用論文

This product has been referenced in:
  • Kanasaki H  et al. Pituitary adenylate cyclase-activating polypeptide (PACAP) increases expression of the gonadotropin-releasing hormone (GnRH) receptor in GnRH-producing GT1-7 cells overexpressing PACAP type I receptor. Gen Comp Endocrinol 193C:95-102 (2013). Read more (PubMed: 23899713) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 1 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (Neurons)
Specification Neurons
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Oct 22 2013

I am very sorry to inform you that the originator of this antigen is unable to supply it. I'm sorry I cannot help you more on this occasion and wish you all the best with your research.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"