Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo.
Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.
Belongs to the polyadenylate-binding protein type-1 family.
Contains 1 PABC domain. Contains 4 RRM (RNA recognition motif) domains.
Arg-518 is dimethylated, probably to asymmetric dimethylarginine.
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Information by UniProt
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has not yet been referenced specifically in any publications.
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