製品の概要

  • 製品名Anti-PABPC4 antibody
    PABPC4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PABPC4
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: AELGAKAKEF TNVYIKNFGE EVDDESLKEL FSQFGKTLSV KVMRDPNGKS, corresponding to a region within amino acids 181-230 of Human PABPC4.

  • ポジティブ・コントロール
    • Human kidney tissue. HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo.
  • 組織特異性Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.
  • 配列類似性Belongs to the polyadenylate-binding protein type-1 family.
    Contains 1 PABC domain.
    Contains 4 RRM (RNA recognition motif) domains.
  • 翻訳後修飾Arg-518 is dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activated platelet protein 1 antibody
    • Activated-platelet protein 1 antibody
    • APP 1 antibody
    • APP-1 antibody
    • APP1 antibody
    • FLJ43938 antibody
    • Inducible poly(A) binding protein antibody
    • Inducible poly(A)-binding protein antibody
    • iPABP antibody
    • IPABP4 antibody
    • PABP 4 antibody
    • PABP-4 antibody
    • PABP4 antibody
    • PABP4_HUMAN antibody
    • PABPC 4 antibody
    • PABPC4 antibody
    • Poly A binding protein cytoplasmic 4 antibody
    • Poly(A) binding protein 4 antibody
    • Poly(A) binding protein cytoplasmic 4 (inducible form) antibody
    • Poly(A) binding protein cytoplasmic 4 antibody
    • Poly(A)-binding protein 4 antibody
    • Polyadenylate binding protein 4 antibody
    • Polyadenylate-binding protein 4 antibody
    • Polyadenylate-binding protein, cytoplasmic, 4 antibody
    • Polyadenylate-binding protein, inducible antibody
    see all

Anti-PABPC4 antibody 画像

  • All lanes : Anti-PABPC4 antibody (ab66897) at 1.25 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PABPC4 with ab66897 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-PABPC4 antibody (ab66897) 使用論文

ab66897 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"