製品の概要

  • 製品名
    Anti-p90 Autoantigen antibody
    p90 Autoantigen 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p90 Autoantigen
  • アプリケーション
    適用あり: WB, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 853-903 of Human p90 Autoantigen (NP_065941.1).

  • ポジティブ・コントロール
    • HeLa and 293T cell lines This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDAMB231

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
  • 組織特異性
    Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
  • 細胞内局在
    Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancerous inhibitor of PP2A antibody
    • Cancerous Inhibitor of Protein Phosphatase 2A antibody
    • CIP2A antibody
    • CIP2A_HUMAN antibody
    • FLJ12850 antibody
    • Kiaa1524 antibody
    • MGC163436 antibody
    • p90 antibody
    • p90 autoantigen antibody
    • Protein CIP2A antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling p90 Autoantigen with ab99518 at 1/200 (1µg/ml). Detection: DAB.
  • ab99518 stained MDAMB231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99518 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-p90 Autoantigen antibody (ab99518) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 102 kDa


    Exposure time : 3 seconds
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99518 at 3 µg/mg lysate; 20% of immunoprecipitate loaded

    Western Blot: p90 Autoantigen antibody (ab99518) at 0.1 µg/ml

    developed using the ECL technique

    Exposure time : 3 seconds

    Predicted band size : 102 kDa

参考文献

ab99518 has not yet been referenced specifically in any publications.

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