Anti-CIP2A 抗体 (ab99518)
Key features and details
- Rabbit polyclonal to CIP2A
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CIP2A antibody
CIP2A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CIP2A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant -
免疫原
Synthetic peptide within Human CIP2A aa 853-903. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HEK293T, HepG2, MCF-7, and HeLa human cell lysates. C6 rat cell lysate. NIH/3T3, CT26, CH27, and TCMK-1 mouse whole cell lysates; IP: HEK293T whole cell lysate; IHC-P: Human colon carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99518の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 102 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation. -
組織特異性
Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level). -
細胞内局在
Membrane. Cytoplasm. Slightly concentrates in the perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 57650 Human
- Entrez Gene: 224171 Mouse
- Entrez Gene: 360711 Rat
- Omim: 610643 Human
- SwissProt: Q8TCG1 Human
- SwissProt: Q8BWY9 Mouse
- Unigene: 591308 Human
- Unigene: 24491 Mouse
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別名
- Cancerous inhibitor of PP2A antibody
- Cancerous Inhibitor of Protein Phosphatase 2A antibody
- CIP2A antibody
see all
画像
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All lanes : Anti-CIP2A antibody (ab99518) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 102 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CIP2A with ab99518 at 1/200 (1 µg/ml). Detection: DAB.
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All lanes : Anti-CIP2A antibody (ab99518) at 0.04 µg/ml
Lane 1 : CT26 (mouse colon carcinoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : CH27 (mouse B cell lymphoma cell line) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 102 kDa
Exposure time: 10 seconds -
Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer labeling CIP2A with ab99518.
Lanes 1-2: Different lots of ab99518 used for IP at 6 µg per reaction.
Lane 3: Control IgG
For blotting immunoprecipitated CIP2A, ab99518 was used at 0.04 µg/ml.
Detection: Chemiluminescence with an exposure time of 1 second.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab99518 は 9 報の論文で使用されています。
- Bownes LV et al. Pre-Clinical Study Evaluating Novel Protein Phosphatase 2A Activators as Therapeutics for Neuroblastoma. Cancers (Basel) 14:N/A (2022). PubMed: 35454859
- Fang D et al. m6A modification-mediated lncRNA TP53TG1 inhibits gastric cancer progression by regulating CIP2A stability. Cancer Sci 113:4135-4150 (2022). PubMed: 36114757
- Yen YT et al. Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response. Nat Commun 12:7297 (2021). PubMed: 34911954
- Williams AP et al. Investigation of PP2A and Its Endogenous Inhibitors in Neuroblastoma Cell Survival and Tumor Growth. Transl Oncol 12:84-95 (2019). PubMed: 30286326
- Stafman LL et al. PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells. PLoS One 14:e0214469 (2019). PubMed: 30969990
- Chiarini A et al. Over Expressed TKTL1, CIP-2A, and B-MYB Proteins in Uterine Cervix Epithelium Scrapings as Potential Risk Predictive Biomarkers in HR-HPV-Infected LSIL/ASCUS Patients. Front Oncol 9:213 (2019). PubMed: 31001477
- Yin J et al. Cip2a/miR-301a feedback loop promotes cell proliferation and invasion of triple-negative breast cancer. J Cancer 10:5964-5974 (2019). PubMed: 31762806
- Lin X et al. FL118 inhibits viability and induces apoptosis of colorectal cancer cells via inactivating the CIP2A/PP2A axis. Life Sci 239:117074 (2019). PubMed: 31751585
- Garner EF et al. FTY720 Decreases Tumorigenesis in Group 3 Medulloblastoma Patient-Derived Xenografts. Sci Rep 8:6913 (2018). PubMed: 29720672