製品の概要

  • 製品名
    Anti-p90 Autoantigen antibody
    p90 Autoantigen 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p90 Autoantigen
  • アプリケーション
    適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 550-600 of Human p90 Autoantigen (NP_065941.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab99396 was affinity purified using an epitope specific to p90 Autoantigen immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
    • 組織特異性
      Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
    • 細胞内局在
      Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cancerous inhibitor of PP2A antibody
      • Cancerous Inhibitor of Protein Phosphatase 2A antibody
      • CIP2A antibody
      • CIP2A_HUMAN antibody
      • FLJ12850 antibody
      • Kiaa1524 antibody
      • MGC163436 antibody
      • p90 antibody
      • p90 autoantigen antibody
      • Protein CIP2A antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling p90 Autoantigen with ab99396 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of p90 Autoantigen by Western Blot of Immunprecipitate.
      anti-p90 Autoantigen at 0.1µg/ml staining p90 Autoantigen in HeLa whole cell lysate immunoprecipitated using ab99396 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    参考文献

    ab99396 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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