製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 8 kDa (predicted molecular weight: 8 kDa).

ターゲット情報

  • 機能Could participate in the response to proapoptotic stimuli and promotes cellular growth in a way that helps the tissue counteract diverse injuries. May contribute to the metastatic phenotype.
  • 組織特異性Highly expressed in pancreas.
  • 翻訳後修飾Phosphorylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Candidate of metastasis 1 antibody
    • COM1 antibody
    • Nuclear protein 1 antibody
    • Nupr1 antibody
    • NUPR1_HUMAN antibody
    • p8 protein antibody
    • Protein p8 antibody
    see all

Anti-p8 antibody (ab6028) 使用論文

ab6028 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human colon cancer cell)
Loading amount 30 µg
Specification human colon cancer cell
Gel Running Conditions Reduced Denaturing (12.5% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 09 2013

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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Thank you for your enquiry. One difference that I see between these two antibodies is that ab6028 has more detail for the immunogen sequence "Synthetic peptide: ERKLVTKLQNSERKKRGARR, corresponding to amino acids 62-82 of Human p8" and ab6027 says that ...

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I'm sorry to hear you are having problem with ab6028. P8 is a nuclear protein, do you do nuclear extraction to have a concentrated sample to load onto your gels? I would like to suggest an overnight incubation of the primary antibody (trying 1:...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"