Anti-p75 NGF Receptor 抗体 [MLR2] (FITC) (ab62122)


  • 製品名
    Anti-p75 NGF Receptor antibody [MLR2] (FITC)
    p75 NGF Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MLR2] to p75 NGF Receptor (FITC)
  • 由来種
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    Reacts with human, mouse and rat. Other species have not yet been tested but it is expected that this antibody will be useful for the study of p75 in primates and other species.
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, ELISAmore details
    適用なし: BL or WB
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Human recombinant p75

  • ポジティブ・コントロール
    • NSC34 motor neuron cell line
  • 特記事項

    The antibody was conjugated to Fluorescein isomer 1 (FITC). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.



Our Abpromise guarantee covers the use of ab62122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt 1/10.

(paraformaldehyde or methanol fixed cells)




ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


ELISA 1/5000.
  • 追加情報
    Is unsuitable for BL or WB.
  • ターゲット情報

    • 機能
      Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
    • 配列類似性
      Contains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • ドメイン
      Death domain is responsible for interaction with RANBP9.
      The extracellular domain is responsible for interaction with NTRK1.
    • 翻訳後修飾
      N- and O-glycosylated.
      O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
      Phosphorylated on serine residues.
    • 細胞内局在
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD271 antibody
      • CD271 antigen antibody
      • Gp80 LNGFR antibody
      • Gp80-LNGFR antibody
      • Low affinity nerve growth factor receptor antibody
      • Low affinity neurotrophin receptor p75NTR antibody
      • Low-affinity nerve growth factor receptor antibody
      • Nerve growth factor receptor antibody
      • Nerve growth factor receptor TNFR superfamily member 16 antibody
      • NGF receptor antibody
      • Ngfr antibody
      • p75 ICD antibody
      • p75 Neurotrophin receptor antibody
      • p75 NTR antibody
      • p75(NTR) antibody
      • p75NTR antibody
      • TNFR Superfamily Member 16 antibody
      • TNFRSF16 antibody
      • TNR16_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 16 antibody
      see all


    • Overlay histogram showing PC12 cells stained with ab62122 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62122, 1/10 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min) used under the same conditions.

      Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • Ab62122 (1-2 ug/ml) intracellular staining of p75NTR in NSC34 motor neuron cell line by live confocal imaging.


    This product has been referenced in:
    • Shen M  et al. Isolation of rat Schwann cells based on cell sorting. Mol Med Rep 16:1747-1752 (2017). Read more (PubMed: 28627700) »
    • Maximino JR  et al. Deregulated expression of cytoskeleton related genes in the spinal cord and sciatic nerve of presymptomatic SOD1(G93A) Amyotrophic Lateral Sclerosis mouse model. Front Cell Neurosci 8:148 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24904291) »

    See all 5 Publications for this product

    レビューと Q&A

    Based on the preferences ab1281 should be the right one.

    Thank you for contacting us.
    I can confirm the isotype controls suggested are suitable for the antibodies requested.
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