製品の概要

  • 製品名
    Anti-p75 NGF Receptor antibody [MLR2]
    p75 NGF Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MLR2] to p75 NGF Receptor
  • アプリケーション
    適用あり: Flow Cyt, ELISA, Competitive ELISA, ICC/IFmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Human p75 coupled to an Fc fragment

  • ポジティブ・コントロール
    • Motor neurons in spinal cord with lesioned sciatic nerve

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab61425 was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS and then lyophilized. Purity was analysed using electrophoresis, 4-12% Bis Tris Gel (Invitrogen).
  • ポリ/モノ
    モノクローナル
  • クローン名
    MLR2
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 20 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA 1/5000.
Competitive ELISA Use at an assay dependent concentration. PubMed: 22292018
ICC/IF Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
    • 配列類似性
      Contains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • ドメイン
      Death domain is responsible for interaction with RANBP9.
      The extracellular domain is responsible for interaction with NTRK1.
    • 翻訳後修飾
      N- and O-glycosylated.
      O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
      Phosphorylated on serine residues.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD271 antibody
      • CD271 antigen antibody
      • Gp80 LNGFR antibody
      • Gp80-LNGFR antibody
      • Low affinity nerve growth factor receptor antibody
      • Low affinity neurotrophin receptor p75NTR antibody
      • Low-affinity nerve growth factor receptor antibody
      • Nerve growth factor receptor antibody
      • Nerve growth factor receptor TNFR superfamily member 16 antibody
      • NGF receptor antibody
      • Ngfr antibody
      • p75 ICD antibody
      • p75 Neurotrophin receptor antibody
      • p75 NTR antibody
      • p75(NTR) antibody
      • p75NTR antibody
      • TNFR Superfamily Member 16 antibody
      • TNFRSF16 antibody
      • TNR16_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 16 antibody
      see all

    Anti-p75 NGF Receptor antibody [MLR2] 画像

    • Ab61425 uptake into mesenteric nerves following in vivo administration into adult rat.

    Anti-p75 NGF Receptor antibody [MLR2] (ab61425) 使用論文

    This product has been referenced in:
    • Uesaka T  et al. Neuronal Differentiation in Schwann Cell Lineage Underlies Postnatal Neurogenesis in the Enteric Nervous System. J Neurosci 35:9879-88 (2015). Read more (PubMed: 26156989) »
    • Zhang C  et al. Suppression of p75 Neurotrophin Receptor Surface Expression with Intrabodies Influences Bcl-xL mRNA Expression and Neurite Outgrowth in PC12 Cells. PLoS One 7:e30684 (2012). Competitive ELISA . Read more (PubMed: 22292018) »

    See all 7 Publications for this product

    Product Wall

    Thanks for your phone call last week and for your patience while I have been in touch with the lab about ab61425. I apologize for the delay in getting back to you. I was told that this antibody was generated against the extracellular domain of ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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