Anti-p75 NGF Receptor 抗体 [MC-192] (ab6172)
Key features and details
- Mouse monoclonal [MC-192] to p75 NGF Receptor
- Suitable for: ICC
- Reacts with: Rat
- Isotype: IgG1
製品の概要
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製品名
Anti-p75 NGF Receptor antibody [MC-192]
p75 NGF Receptor 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MC-192] to p75 NGF Receptor -
由来種
Mouse -
アプリケーション
適用あり: ICCmore details -
種交差性
交差種: Rat -
免疫原
Tissue, cells or virus corresponding to Rat p75 NGF Receptor. PC-12 cells.
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ポジティブ・コントロール
- This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.
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特記事項
A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor. -
ポリ/モノ
モノクローナル -
クローン名
MC-192 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6172の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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特記事項 |
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ICC
Use at an assay dependent concentration. |
ターゲット情報
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機能
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
配列類似性
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
ドメイン
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
翻訳後修飾
N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 24596 Rat
- SwissProt: P07174 Rat
- Unigene: 10980 Rat
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別名
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
画像
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ab6172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6172 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (13)
ab6172 は 13 報の論文で使用されています。
- Molnár K et al. Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury. J Neuroinflammation 19:68 (2022). PubMed: 35305649
- Rogdakis T et al. Development and Biological Characterization of a Novel Selective TrkA Agonist with Neuroprotective Properties against Amyloid Toxicity. Biomedicines 10:N/A (2022). PubMed: 35327415
- Niapour N et al. An efficient system for selection and culture of Schwann cells from adult rat peripheral nerves. Cytotechnology 68:629-36 (2016). PubMed: 25680695
- Nelson AR et al. Noradrenergic sympathetic sprouting and cholinergic reinnervation maintains non-amyloidogenic processing of AßPP. J Alzheimers Dis 38:867-79 (2014). IHC ; Rat . PubMed: 24081376
- Barres BA Designing and troubleshooting immunopanning protocols for purifying neural cells. Cold Spring Harb Protoc 2014:1342-7 (2014). PubMed: 25447277