製品の概要

  • 製品名
    Anti-p75 NGF Receptor antibody
    p75 NGF Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p75 NGF Receptor
  • 特異性
    ab25958 recognises NGFR (p75NTR, TNFRSF16).
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length human NGFR

  • ポジティブ・コントロール
    • A-20 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • 配列類似性
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • ドメイン
    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • 翻訳後修飾
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence of HCEM cells labelling p75 NGF Receptor (green) with ab25958 at 1/500. Cells were cultured until low confluence on 35mm glass base dishes and washed with PBS. Cells were fixed with 4% paraformaldehyde in PBS and blocked by incubation with TBS containing 0.2% casein and 0.1% Triton X-100 for 20 minutes. Cells were incubated with primary antibody (1/500) overnight at 4°C. An Alexa Fluor® 488 anti-rabbit IgG (1/200) was used as the secondary antibody. Beta actin was stained with Alexa Fluor® 594 phalloidin (red).

  • ab25958 at 10µg/ml staining p75NGF Receptor in A20 cells by ICC/IF

参考文献

This product has been referenced in:
  • Kajiya M  et al. Brain-derived neurotrophic factor stimulates bone/cementum-related protein gene expression in cementoblasts. J Biol Chem 283:16259-67 (2008). WB, ICC/IF ; Human . Read more (PubMed: 18390540) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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