製品の概要

  • 製品名
    Anti-p75 NGF Receptor antibody [2E3]
    p75 NGF Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [2E3] to p75 NGF Receptor
  • アプリケーション
    適用あり: ICC/IF, IP, WB, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation: mouse embryo kidney.

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/1000.
IP 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 75 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/10.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • 配列類似性
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • ドメイン
    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • 翻訳後修飾
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

画像

  • Anti-p75 NGF Receptor antibody [2E3] (ab27007) + BW5147 NGFR expression cells lysate

    Secondary
    anti-rat secondary antibody conjugated to HRP

    Predicted band size : 75 kDa
    Observed band size : 75 kDa
    A chemiluminescence detection system was used.
  • Mouse embryo kidney immunostaining by ab27007.
  • Detection of cell membrane mouse Endomucin in BW5147 cell lines. Cells were stained using ab27007 and anti-rat IgG FITC conjugated mAb(blue) or the isotype control(red). Flow cytometry was performed on a FACSCaliburTM.

参考文献

This product has been referenced in:
  • Lopes CS  et al. CD271+ Mesenchymal Stem Cells as a Possible Infectious Niche for Leishmania infantum. PLoS One 11:e0162927 (2016). ICC/IF ; Mouse . Read more (PubMed: 27622907) »
  • Garhyan J  et al. Preclinical and Clinical Evidence of Mycobacterium tuberculosis Persistence in the Hypoxic Niche of Bone Marrow Mesenchymal Stem Cells after Therapy. Am J Pathol 185:1924-34 (2015). Read more (PubMed: 26066709) »

See all 3 Publications for this product

レビューと Q&A

Thank you for your enquiry. Our short answer is that ab27007 was raised against the whole receptor and not a portion of it, and that the region of the receptor that the antibody binds has not been mapped. Production of the antibody is described...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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