Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma)
Specification
Melanoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
Permeabilization
Yes - 0.25%Triton X-100
Blocking step
DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor594 Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/100
Additional data
Additional Notes
Permeabilization is not necessary but I have to do it because I need to stain another antigen that present in cytoplasm.
Red: p75 positive cells.
Green: tumor cells.
Blue: DAPI stained nucleus.
Red: p75 positive cells.
Green: tumor cells.
Blue: DAPI stained nucleus.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Hongwei Shao
Verified customer
投稿 Nov 09 2011