製品の概要

  • 製品名Anti-p73 (phospho Y99) antibody
    p73 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p73 (phospho Y99)
  • 特異性ab38457 recognises endogenous levels of p73 only when phosphorylated at tyrosine 99.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human p73 around the phosphorylation site of tyrosine 99 (SPYpAQ).

  • ポジティブ・コントロール
    • Human lung carcinoma tissue, extracts from K562 cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab38457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 70 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
  • 組織特異性Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
  • 配列類似性Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • ドメインPossesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
    The WW-binding motif mediates interaction with WWOX.
  • 翻訳後修飾Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
    Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
    Ubiquitinated; leading to its degradation by the proteasome.
  • 細胞内局在Nucleus. Accumulates in the nucleus in response to DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • p53 like transcription factor antibody
    • p53 related protein antibody
    • p53-like transcription factor antibody
    • p53-related protein antibody
    • p73 antibody
    • P73_HUMAN antibody
    • TP73 antibody
    • Tumor protein p73 antibody
    see all

Anti-p73 (phospho Y99) antibody 画像

  • All lanes : Anti-p73 (phospho Y99) antibody (ab38457) at 1/500 dilution

    Lane 1 : untreated extracts from K562 cells
    Lane 2 : Pervanadate treated extracts from K562 cells

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 70 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of p73 (phospho Y99) in paraffin embedded human lung carcinoma tissue sections using 1/50 ab38457. Left: untreated sample. Right: sample pretreated with phosphopeptide (negative control).

Anti-p73 (phospho Y99) antibody (ab38457) 使用論文

ab38457 has not yet been referenced specifically in any publications.

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