製品の概要

  • 製品名
    Anti-p73 antibody - ChIP Grade
    p73 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p73 - ChIP Grade
  • 特異性
    The antibody is not expected to react with deltaNp73.
  • アプリケーション
    適用あり: WB, IP, IHC-P, ChIPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, African green monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human p73 aa 1-62. The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73.
    Database link: O15350

  • ポジティブ・コントロール
    • 293T, MCF7 and COS-1 cells.
  • 特記事項
    Centrifuge tube to remove product from lid. This antibody has ben tested in ChIP at an inducible promotor held on a vector. There was very siginifant enrichment following induction at the locus analysed. This antibody works well in ChIP in this assay.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2500 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).
IP Use at 1-4 µg/mg of lysate.
IHC-P Use at an assay dependent concentration. PubMed: 16630058
ChIP Use at an assay dependent concentration. PubMed: 21459846

ターゲット情報

  • 機能
    Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
  • 組織特異性
    Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
  • 配列類似性
    Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • ドメイン
    Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
    The WW-binding motif mediates interaction with WWOX.
  • 翻訳後修飾
    Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
    Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
    Ubiquitinated; leading to its degradation by the proteasome.
  • 細胞内局在
    Nucleus. Accumulates in the nucleus in response to DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • p53 like transcription factor antibody
    • p53 related protein antibody
    • p53-like transcription factor antibody
    • p53-related protein antibody
    • p73 antibody
    • P73_HUMAN antibody
    • TP73 antibody
    • Tumor protein p73 antibody
    see all

参考文献

This product has been referenced in:
  • Ratovitski EA Tumor Protein (TP)-p53 Members as Regulators of Autophagy in Tumor Cells upon Marine Drug Exposure. Mar Drugs 14:N/A (2016). Read more (PubMed: 27537898) »
  • Kramer D  et al. A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity. Cell Death Dis 6:e1634 (2015). ChIP . Read more (PubMed: 25675294) »

See all 9 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (medullary thyroid cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Epitope retrieval solution pH9, 1x
Permeabilization
No
Specification
medullary thyroid cancer
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 50% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 29 2016

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Mouse Cell lysate - nuclear (mouse embrionic stem cell)
Specification
mouse embrionic stem cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 02 2013

Thank you for contacting us.

Checking the datasheet, we have a positive Abreviews in which the customers used human choroid plexus lysateand cerebrospinal fluid in both ICC and WB:

http://www.abcam.com/index.html?datasheet=14430&am...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (human cancer cell lines)
Specification
human cancer cell lines
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
stable Saos cell line with inducible overexpression of TAp73alpha and Tap73beta
Negative control
Saos parental cells and Saos cells overexpressing p53
Username

Mrs. Daniela Kramer

Verified customer

投稿 May 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (CSF)
Loading amount
50 µg
Specification
CSF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Alfredo Cabrera

Verified customer

投稿 Dec 11 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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