Anti-P70 S6 Kinase beta 抗体 (ab70271)

製品の概要

  • 製品名
    Anti-P70 S6 Kinase beta antibody
    P70 S6 Kinase beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to P70 S6 Kinase beta
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between C terminal amino acids 430-482 of Human P70 S6 Kinase beta (NP_003943.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-6 µg/mg of lysate.

ターゲット情報

  • 機能
    Phosphorylates specifically ribosomal protein S6.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 70 kDa ribosomal protein S6 kinase 2 antibody
    • EC 2.7.11.1 antibody
    • KS6B2_HUMAN antibody
    • p70 beta antibody
    • p70 ribosomal S6 kinase beta antibody
    • p70 S6 kinase beta antibody
    • p70 S6K-beta antibody
    • p70 S6KB antibody
    • p70 S6Kbeta antibody
    • p70(S6K) beta antibody
    • p70-beta antibody
    • p70-S6K 2 antibody
    • P70S6K2 antibody
    • p70S6Kb antibody
    • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
    • Ribosomal protein S6 kinase B2 antibody
    • Ribosomal protein S6 kinase beta 2 antibody
    • Ribosomal protein S6 kinase beta-2 antibody
    • Rps6kb2 antibody
    • S6 kinase related kinase antibody
    • S6 kinase-related kinase antibody
    • S6K beta 2 antibody
    • S6K beta antibody
    • S6K-beta antibody
    • S6K-beta-2 antibody
    • S6K2 antibody
    • Serine/threonine protein kinase 14 beta antibody
    • Serine/threonine-protein kinase 14B antibody
    • SRK antibody
    • STK14B antibody
    see all

画像

  • All lanes : Anti-P70 S6 Kinase beta antibody (ab70271) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 15 µg
    Lane 2 : HeLa whole cell lysate at 5 µg


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 35 kDa,67 kDa,69 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

参考文献

ab70271 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for the additional protocol details and for the image provided. Have you tried any shorter incubations with the primary, such as 1 hr? Although we normally recommend ON for the strongest signal, reducing this time may eliminate some of the ba...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録