製品の概要

  • 製品名Anti-p66 beta antibody
    p66 beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p66 beta
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1 and 50 of human p66 beta (NP_065750.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
  • 組織特異性Ubiquitous.
  • 配列類似性Contains 1 GATA-type zinc finger.
  • ドメインCR1 is required for interaction with MBD3 and transcription repression.
    CR2 is required for localization to discrete loci in the nucleus.
  • 細胞内局在Nucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ37346 antibody
    • GATA zinc finger domain containing 2B antibody
    • GATA zinc finger domain containing protein 2B antibody
    • GATA zinc finger domain-containing protein 2B antibody
    • GATAD2B antibody
    • KIAA1150 antibody
    • MGC138257 antibody
    • MGC138285 antibody
    • mKIAA1150 antibody
    • p66/p68 antibody
    • P66B_HUMAN antibody
    • P66beta antibody
    • RGD1308533 antibody
    • transcription repressor p66 beta component of the MeCP1 complex antibody
    • Transcriptional repressor p66 beta antibody
    • Transcriptional repressor p66-beta antibody
    see all

Anti-p66 beta antibody 画像

  • All lanes : Anti-p66 beta antibody (ab76924) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 66 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of p66 beta by Western Blot of Immunprecipitate.
    ab76924 at 1µg/ml staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab76924 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-p66 beta antibody (ab76924) 使用論文

ab76924 has not yet been referenced specifically in any publications.

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