Anti-GATAD2B 抗体 (ab76924)
Key features and details
- Rabbit polyclonal to GATAD2B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GATAD2B antibody
GATAD2B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GATAD2B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant -
免疫原
Synthetic peptide corresponding to Human GATAD2B aa 1-50 (N terminal).
Database link: NP_065750.1 -
ポジティブ・コントロール
- WB: HeLa, 293T and CT26-WT whole cell lysates. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76924の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 GATA-type zinc finger. -
ドメイン
CR1 is required for interaction with MBD3 and transcription repression.
CR2 is required for localization to discrete loci in the nucleus. -
細胞内局在
Nucleus. Nuclear, in discrete foci. - Information by UniProt
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参照データベース
- Entrez Gene: 57459 Human
- Entrez Gene: 229542 Mouse
- Entrez Gene: 310614 Rat
- Omim: 614998 Human
- SwissProt: Q8WXI9 Human
- SwissProt: Q8VHR5 Mouse
- Unigene: 4779 Human
- Unigene: 596854 Human
see all -
別名
- FLJ37346 antibody
- GATA zinc finger domain containing 2B antibody
- GATA zinc finger domain containing protein 2B antibody
see all
画像
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All lanes : Anti-GATAD2B antibody (ab76924) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 66 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
All lanes : Anti-GATAD2B antibody (ab76924) at 1/2000 dilution
Lane 1 : NIH 3T3 (Mouse embryonic fibroblasts) whole cell lysate
Lane 2 : TCMK-1 (Transformed C3H Mouse Kidney-1) whole cell lysate
Lane 3 : CT26-WT (Mouse colon carcinoma cell - Wild Type) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of GATAD2B by Western Blot of Immunprecipitate.
ab76924 at 1µg/ml staining GATAD2B in HeLa whole cell lysate immunoprecipitated using ab76924 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab76924 は 2 報の論文で使用されています。
- Chalei V et al. The long non-coding RNA Dali is an epigenetic regulator of neural differentiation. Elife 3:e04530 (2014). PubMed: 25415054
- Cui S et al. Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic {beta}-Type Globin Promoters in Differentiated Adult Erythroid Cells. Mol Cell Biol 31:3298-311 (2011). PubMed: 21670149