製品の概要

  • 製品名Anti-p66 alpha antibody
    p66 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p66 alpha
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 573 - 633 of Human p66 alpha (AAO31797.1)

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab87663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Predicted molecular weight: 68 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Transcriptional repressor.
  • 組織特異性Ubiquitous, both in fetal and adult tissues.
  • 配列類似性Contains 1 GATA-type zinc finger.
  • 細胞内局在Nucleus speckle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ20085 antibody
    • FLJ21017 antibody
    • GATA zinc finger domain containing 2A antibody
    • GATA zinc finger domain containing protein 2A antibody
    • GATA zinc finger domain-containing protein 2A antibody
    • GATAD2A antibody
    • Hp66 alpha antibody
    • Hp66alpha antibody
    • OTTHUMP00000201911 antibody
    • OTTHUMP00000201912 antibody
    • P66 alpha antibody
    • P66A_HUMAN antibody
    • p66alpha antibody
    • Transcriptional repressor p66 alpha antibody
    • Transcriptional repressor p66-alpha antibody
    • Transcriptional repressor p66alpha antibody
    see all

Anti-p66 alpha antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling p66 alpha with ab87663 at 1/1000 (1µg/ml). Detection: DAB.
  • ab87663 staining p66 alpha in mouse MEL cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200 in TBS) for 12 hours at 4°C. An FITC-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-p66 alpha antibody (ab87663) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml

    Developed using the ECL technique

    Predicted band size : 68 kDa


    Exposure time : 1 minute
  • Detection of p66 alpha by Western Blot of Immunprecipitate. ab87663 at 1µg/ml staining p66 alpha in HeLa whole cell lysate immunoprecipitated using ab87663 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-p66 alpha antibody (ab87663) 使用論文

ab87663 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (MEL)
Specification MEL
Permeabilization Yes - tween-20
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 May 14 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample Mouse Tissue sections (kidney)
Specification kidney
Permeabilization No
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Dec 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (murine erythroleukemia (MEL cell))
Loading amount 30 µg
Specification murine erythroleukemia (MEL cell)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
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Dr. Min young Kim

Verified customer

投稿 Dec 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"