製品の概要

  • 製品名Anti-p63 antibody [4E5]
    p63 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4E5] to p63
  • アプリケーション適用あり: Flow Cyt, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide of Human p63.

  • ポジティブ・コントロール
    • A431, HeLa, Jurkat, THP1, NIH 3T3, COS7 and PC12 cell lysates. Ovarian cancer and lung cancer tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 77 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.

ターゲット情報

  • 機能Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.
  • 組織特異性Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
  • 関連疾患Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting.
    Defects in TP63 are the cause of ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]. AEC is an autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiform adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate.
    Defects in TP63 are the cause of ectrodactyly-ectodermal dysplasia-cleft lip/palate syndrome type 3 (EEC3) [MIM:604292]. EEC3 is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting.
    Defects in TP63 are the cause of split-hand/foot malformation type 4 (SHFM4) [MIM:605289]. Split-hand/split-foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. There is restricted overlap between the mutational spectra of EEC3 and SHFM4.
    Defects in TP63 are the cause of limb-mammary syndrome (LMS) [MIM:603543]. LMS is characterized by ectrodactyly, cleft palate and mammary-gland abnormalities.
    Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and ovarian cancers.
    Defects in TP63 are a cause of ectodermal dysplasia Rapp-Hodgkin type (EDRH) [MIM:129400]; also called Rapp-Hodgkin syndrome or anhidrotic ectodermal dysplasia with cleft lip/palate. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDRH is characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate.
    Defects in TP63 are the cause of non-syndromic orofacial cleft type 8 (OFC8) [MIM:129400]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • 配列類似性Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • ドメインThe transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
  • 翻訳後修飾May be sumoylated.
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIS antibody
    • Amplified in squamous cell carcinoma antibody
    • B(p51A) antibody
    • B(p51B) antibody
    • Chronic ulcerative stomatitis protein antibody
    • CUSP antibody
    • DN p63 alpha 1 antibody
    • DNp63 antibody
    • EEC3 antibody
    • id:ibd3516 antibody
    • Keratinocyte transcription factor antibody
    • Keratinocyte transcription factor KET antibody
    • KET antibody
    • LMS antibody
    • MGC115972 antibody
    • MGC192897 antibody
    • NBP antibody
    • OFC8 antibody
    • OTTHUMP00000209732 antibody
    • OTTHUMP00000209733 antibody
    • OTTHUMP00000209734 antibody
    • OTTHUMP00000209735 antibody
    • OTTHUMP00000209737 antibody
    • OTTHUMP00000209738 antibody
    • OTTHUMP00000209739 antibody
    • OTTHUMP00000209740 antibody
    • OTTHUMP00000209741 antibody
    • OTTHUMP00000209742 antibody
    • OTTHUMP00000209743 antibody
    • OTTHUMP00000209744 antibody
    • p40 antibody
    • p51 antibody
    • P51/P63 antibody
    • p53-related protein p63 antibody
    • p53CP antibody
    • p63 antibody
    • P63_HUMAN antibody
    • p73H antibody
    • p73L antibody
    • RHS antibody
    • SHFM4 antibody
    • TAp63alpha antibody
    • TP53CP antibody
    • TP53L antibody
    • TP63 antibody
    • TP73L antibody
    • Transformation related protein 63 antibody
    • Transformation-related protein 63 antibody
    • Trp53rp1 antibody
    • Trp63 antibody
    • Tumor protein 63 antibody
    • Tumor protein p53-competing protein antibody
    • Tumor protein p53-like antibody
    • Tumor protein p63 antibody
    • Tumor protein p63 deltaN isoform delta antibody
    • Tumor protein p73 antibody
    • Tumor protein p73-like antibody
    see all

Anti-p63 antibody [4E5] 画像

  • All lanes : Anti-p63 antibody [4E5] (ab110038) at 1/500 dilution

    Lane 1 : A431 cell lysate.
    Lane 2 : HeLa cell lysate.
    Lane 3 : Jurkat cell lysate.
    Lane 4 : THP1 cell lysate.
    Lane 5 : NIH 3T3 cell lysate.
    Lane 6 : COS7 cell lysate.
    Lane 7 : PC12 cell lysate.


    Predicted band size : 77 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
  • ab110038 should be used at a proposed dilution of 1/10000 for ELISA.
  • ab110038 at 1/200 dilution staining p63 in paraffin-embedded ovarian cancer tissue by Immunohistochemistry. Detection utilised DAB staining.
  • ab110038 at 1/200 dilution staining p63 in paraffin-embedded lung cancer tissue by Immunohistochemistry. Detection utilised DAB staining.
  • Overlay histogram showing A431 cells stained with ab110038 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110038, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-p63 antibody [4E5] (ab110038) 使用論文

ab110038 has not yet been referenced specifically in any publications.

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We have not done the relevant testing in order to identify if the antibody is specific to Delta Np63 alpha.

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I can confirm that you could use the EasyLink conjugation kit to conjguate ab110038 to use in flow cystometry. However, as ab110038 is sold as ascites fluid, you would need to purify the antibody first. For this, ...

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DISCOUNT CODE: *** Expiration date: April 16, 2011 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab110038 in flow cytometry and include this code in the “Additional Comme...

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