製品の概要

  • 製品名Anti-p63 antibody [4A4]
    p63 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4A4] to p63
  • 特異性The antibody binds all of the forms of p63 (TA and deltaN).
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, Turtle
  • 免疫原

    Recombinant fragment corresponding to Human p63 aa 1-205. This antibody is raised against the N-terminus of ∆N p63 isoform of mouse protein.
    Database link: Q9H3D4-2

  • ポジティブ・コントロール
    • Normal prostate.
  • 特記事項

    Please note that clone [BC4A4] and clone [4A4] are identical.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50. PubMed: 15800938
IHC-P 1/40 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See Sanderson et al and Huange et al. or the Abreview 22860.

Alternatively, steam tissue sections for 45-60 minutes. Allow solution to cool for 10 minutes then wash in distilled water.

 

IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.
  • 組織特異性Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
  • 関連疾患Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting.
    Defects in TP63 are the cause of ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]. AEC is an autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiform adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate.
    Defects in TP63 are the cause of ectrodactyly-ectodermal dysplasia-cleft lip/palate syndrome type 3 (EEC3) [MIM:604292]. EEC3 is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting.
    Defects in TP63 are the cause of split-hand/foot malformation type 4 (SHFM4) [MIM:605289]. Split-hand/split-foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. There is restricted overlap between the mutational spectra of EEC3 and SHFM4.
    Defects in TP63 are the cause of limb-mammary syndrome (LMS) [MIM:603543]. LMS is characterized by ectrodactyly, cleft palate and mammary-gland abnormalities.
    Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and ovarian cancers.
    Defects in TP63 are a cause of ectodermal dysplasia Rapp-Hodgkin type (EDRH) [MIM:129400]; also called Rapp-Hodgkin syndrome or anhidrotic ectodermal dysplasia with cleft lip/palate. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDRH is characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate.
    Defects in TP63 are the cause of non-syndromic orofacial cleft type 8 (OFC8) [MIM:129400]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • 配列類似性Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • ドメインThe transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
  • 翻訳後修飾May be sumoylated.
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIS antibody
    • Amplified in squamous cell carcinoma antibody
    • B(p51A) antibody
    • B(p51B) antibody
    • Chronic ulcerative stomatitis protein antibody
    • CUSP antibody
    • DN p63 alpha 1 antibody
    • DNp63 antibody
    • EEC3 antibody
    • id:ibd3516 antibody
    • Keratinocyte transcription factor antibody
    • Keratinocyte transcription factor KET antibody
    • KET antibody
    • LMS antibody
    • MGC115972 antibody
    • MGC192897 antibody
    • NBP antibody
    • OFC8 antibody
    • OTTHUMP00000209732 antibody
    • OTTHUMP00000209733 antibody
    • OTTHUMP00000209734 antibody
    • OTTHUMP00000209735 antibody
    • OTTHUMP00000209737 antibody
    • OTTHUMP00000209738 antibody
    • OTTHUMP00000209739 antibody
    • OTTHUMP00000209740 antibody
    • OTTHUMP00000209741 antibody
    • OTTHUMP00000209742 antibody
    • OTTHUMP00000209743 antibody
    • OTTHUMP00000209744 antibody
    • p40 antibody
    • p51 antibody
    • P51/P63 antibody
    • p53-related protein p63 antibody
    • p53CP antibody
    • p63 antibody
    • P63_HUMAN antibody
    • p73H antibody
    • p73L antibody
    • RHS antibody
    • SHFM4 antibody
    • TAp63alpha antibody
    • TP53CP antibody
    • TP53L antibody
    • TP63 antibody
    • TP73L antibody
    • Transformation related protein 63 antibody
    • Transformation-related protein 63 antibody
    • Trp53rp1 antibody
    • Trp63 antibody
    • Tumor protein 63 antibody
    • Tumor protein p53-competing protein antibody
    • Tumor protein p53-like antibody
    • Tumor protein p63 antibody
    • Tumor protein p63 deltaN isoform delta antibody
    • Tumor protein p73 antibody
    • Tumor protein p73-like antibody
    see all

Anti-p63 antibody [4A4] 画像

  • Immunohistochemical analysis of prostate tissue labelling p63 with ab735.

  • Anti-p53 antibody (ab735) staining p63 in murine mammary gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 4% serum for 1 hour at 22°C followed by incubation with the primary antibody at a 1/40 dilution for 15 hours at 4°C. A Biotin-conjugated anti-mouse polyclonal was used as secondary antibody at a 1/250 dilution. Note positive nuclear staining of the basal cell population.

    See Abreview

Anti-p63 antibody [4A4] (ab735) 使用論文

This product has been referenced in:
  • Hall JS  et al. Investigation of radiosensitivity gene signatures in cancer cell lines. PLoS One 9:e86329 (2014). WB ; Human . Read more (PubMed: 24466029) »
  • Veraitch O  et al. Human induced pluripotent stem cell-derived ectodermal precursor cells contribute to hair follicle morphogenesis in vivo. J Invest Dermatol 133:1479-88 (2013). Read more (PubMed: 23321923) »

See all 8 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (Epidermal keratinocytes)
Negative control Negative control loci: Myoglobin exon 2 region, Chr11 intergenic region
Specification Epidermal keratinocytes
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Positive control Positive control loci: p21 promoter, BPAG1, MiR-205, IRF6
Username

Jo Zhou

Verified customer

投稿 Dec 30 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - nuclear (Normal Human Keratinocyte)
Specification Normal Human Keratinocyte
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Mar 30 2015

There are many isoforms of different sizes. Twelve are listed at the UniProt entry for human p63.

http://www.uniprot.org/uniprot/Q9H3D4

The molecular weights listed there (you will need to scroll far down the page) are calculated...

Read More

For ab735, anti-p63 (currently unpublished), we do not have any western blot data but we did at one point offer this same clone, 4A4 (referred to on the ab735 datasheet as BC4A4), as a different catalogue item, ab3239. That product was used in the foll...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Breast)
Specification Breast
Fixative Formaldehyde
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Rene Villadsen

Verified customer

投稿 Jul 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Turtle Tissue sections (normal skin)
Specification normal skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8 30min
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Apr 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (normal skin)
Specification normal skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8 30min
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Apr 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mammary gland)
Specification Mammary gland
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"