Anti-p60 CAF1/MPP7 抗体 (ab72520)
Key features and details
- Rabbit polyclonal to p60 CAF1/MPP7
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-p60 CAF1/MPP7 antibody
p60 CAF1/MPP7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p60 CAF1/MPP7 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human p60 CAF1/MPP7 (C terminal).
Database link: NP_005432.1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72520の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Detects a band of approximately 25, 65, 130, 240 kDa (predicted molecular weight: 61 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 25, 65, 130, 240 kDa (predicted molecular weight: 61 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. -
配列類似性
Belongs to the WD repeat HIR1 family.
Contains 7 WD repeats. -
発生段階
Active complex is found in G1, S and G2 phases. -
翻訳後修飾
Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. -
細胞内局在
Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase. - Information by UniProt
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参照データベース
- Entrez Gene: 8208 Human
- Omim: 601245 Human
- SwissProt: Q13112 Human
- Unigene: 75238 Human
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別名
- CAF 1 subunit B antibody
- CAF 1A antibody
- CAF I 60 kDa subunit antibody
see all
画像
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All lanes : Anti-p60 CAF1/MPP7 antibody (ab72520) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 240 kDa, 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling p60 CAF1/MPP7 with ab7252 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation/ Western Blot of p60 CAF1/MPP7. Lane 1: ab72520 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72520 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab72520 は 2 報の論文で使用されています。
- Gong L et al. Ubiquitin ligase CHAF1B induces cisplatin resistance in lung adenocarcinoma by promoting NCOR2 degradation. Cancer Cell Int 20:194 (2020). PubMed: 32508530
- Yang C et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res 45:739-748 (2017). PubMed: 27794043