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Our Abpromise guarantee covers the use of ab4058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17709399|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
ab4058 staining mouse E14.5 embryonic eye tissue sections by IHC-Fr. Sections were PFA fixed and blocked with 1% BSA for 2 hours at 25°C prior to incubating with ab4058, diluted 1/100, for 12 hours at 25°C. A FITC conjugated goat anti-rabbit antibody, diluted 1/400 was used as the secondary.