Anti-p57 Kip2 (phospho T310) 抗体 (ab61064)

製品の概要

  • 製品名
    Anti-p57 Kip2 (phospho T310) antibody
    p57 Kip2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to p57 Kip2 (phospho T310)
  • 特異性
    Ab61064 detects endogenous levels of p57 Kip2 only when phosphorylated at threonine 310.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized phosphopeptide derived from human p57 Kip2 around the phosphorylation site of threonine 310(E-Q-TP-P-R).

  • ポジティブ・コントロール
    • Human placenta tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab61064 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
  • 組織特異性
    Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
  • 関連疾患
    Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
    Note=Defects in CDKN1C are involved in tumor formation.
  • 配列類似性
    Belongs to the CDI family.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beckwith Wiedemann syndrome antibody
    • BWCR antibody
    • BWS antibody
    • CDKI antibody
    • CDKN 1C antibody
    • CDKN1C antibody
    • CDN1C_HUMAN antibody
    • Cyclin dependent kinase inhibitor 1C antibody
    • Cyclin dependent kinase inhibitor p57 antibody
    • Cyclin-dependent kinase inhibitor 1C antibody
    • Cyclin-dependent kinase inhibitor p57 antibody
    • KIP 2 antibody
    • KIP2 antibody
    • p57 antibody
    • p57 Kip 2 antibody
    • p57KIP2 antibody
    • WBS antibody
    see all

画像

  • Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab61064 at 1/50-1/100 dilution with or without addition of Phospho-peptide.


  • Predicted band size : 32 kDa

    Western Blot analysis of K562 cell lysates labeling p57 Kip2 (phospho T310) with ab61064 at 0.01U/ml for 15 minutes. The lane on the right is blocked with the phospho peptide.

参考文献

ab61064 has not yet been referenced specifically in any publications.

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